Mesenchymal precursor cell

Details Mesenchymal precursor cell Mesenchymal precursor cell

2006-10-17

2006-10-17

Chan, Christina (Department: 1644)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

C424S577000, C435S325000, C435S372000

Reexamination Certificate

active

07122178

ABSTRACT:
A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

REFERENCES:
patent: 5197985 (1993-03-01), Caplan et al.
patent: 5226914 (1993-07-01), Caplan et al.
patent: 5486359 (1996-01-01), Caplan et al.
patent: 5591625 (1997-01-01), Gerson et al.
patent: 5811094 (1998-09-01), Caplan
patent: 5827740 (1998-10-01), Pittenger
patent: 5837539 (1998-11-01), Caplan et al.
patent: 5922597 (1999-07-01), Verfaillie et al.
patent: 5980887 (1999-11-01), Isner et al.
patent: 6022540 (2000-02-01), Bruder et al.
patent: 6087113 (2000-07-01), Caplan et al.
patent: 6261549 (2001-07-01), Fernandez et al.
patent: 6322784 (2001-11-01), Pittenger et al.
patent: 6335195 (2002-01-01), Rodgers et al.
patent: 6387367 (2002-05-01), Davis-Sproul et al.
patent: 6387369 (2002-05-01), Pittenger et al.
patent: 6645727 (2003-11-01), Thomas et al.
patent: 6709864 (2004-03-01), Pittenger et al.
patent: 2002/0085996 (2002-07-01), McIntosh et al.
patent: 2004/0107453 (2004-06-01), Furcht et al.
patent: WO 01011011 (2001-02-01), None
Gronthos et al., J of Cell Science 2003, V. 116, pp. 1827-1835.
Simmons, P.J., et al., “Isolation, Characterization and Functional Activity of Human Marrow Stromal Progenitors in Hemopoiesis,”Advances in Bone Marrow Purging and Processing: Fourth International Symposium, 1994, pp. 271-280.
Gronthos, S., et al., “The STRO-1+Fraction of Adult Human Bone Marrow Contains the Osteogenic Precursors,”Blood, vol. 84, No. 12, 1994, pp. 4164-4173.
Allen, T.D.: Haemopoietic microenvironment in vitro: Ultrastructural aspects. In Porter R. Whelan J (eds); “Microenvironments in haemopoietic and lymphoid differentiation.” CIBA Foundation Symposium 84. London: Pitman Medical, 1981, pp. 38-67.
Allen et al (1990a) Immunol. Ser. 49:1.
Allen et al (1990b) Marrow Biology and Stem Cells. In Colony Stimulating Factors: Molecular and Cellular Biology, edited by T.M. Dexter, J.M. Garland and N.G. Testa: New York: Marcel Decker, 1990b. pp. 1-38.
Anklesaria et al (1989) Blood. 74:1144.
Anklesaria et al (1987) Proc. Natl. Acad. Sci. USA. 84:7681.
Bennett et al (1991) Journal of Cell Science. 99:131.
Bentley (1982) Br J Haematol. 50:1.
Castro-Malaspina et al (1980) Blood. 56:289.
Castro-Malaspina et al. (1981) Blood. 57:781.
Dexter et al. (1977) J Cell Physiol. 91:335.
Dexter et al Kroc Foundation Series vol. 18; Alan R. Liss, Inc., New York; p. 57-96. 1984.
Fong et al (1997) BioTechniques. 23:1029.
Friedenstein (1976) International Review of Cytology. 47:327.
Friedenstein et al (1970) Cell Tissue Kinetics. 3:393.
Friedenstein et al. (1992) Bone and Mineral. 18:199.
Friedenstein (1980) “Stromal mechanisms of bone marrow: cloning in vitro and retransplantation in vivo.” Immunology of Bone Marrow Transplantation; Ed: S. Thienfelder; Springer-Verlag Berlin; p. 19-29, 1980.
Gronthos et al. (1994) Blood. 84:4164.
Gronthos and Simmons (1995) Blood. 85:929.
Huang and Terstappen (1992) Nature. 360:745.
Keating et al. (1982) Nature. 298:280.
Kim et al. (1994) Science, 266:2011.
Knopse et al. (1966) Blood. 28:398.
Knopse et al. (1972) Blood. 39:331.
Lichtman (1981) Experimental Hematology. 9:391.
Long (1992) Exp Hematol. 20:288.
MacManus and Weiss (1984) Blood. 64:1036.
McIntyre and Bjornson (1986) Exp Hematol. 14:833.
Miltenyi et al. (1990) Cytometry. 11:231.
Owen (1985) Bone and Mineral Research. 3:1.
Owen and Friedenstein (1988) CIBA Foundation symposium. 136:42.
Perkins and Fleischman (1990) Blood. 75:620.
Piersma et al. (1983) Br. J. Haematol. 54:285.
Rothstein et al. (1985) Blood. 65:744.
Simmons and Gronthos. (1991) International Journal of Cell Cloning. 9:408 [abstract].
Simmons et al.(1994) Advances in Bone Marrow Purging and Processing: Fourth International Symposium. Progress in Clinical Biological Research. 389:271.
Simmons et al. (1987) Nature 328:429.
Simmons and Torok-Storb (1991a) Blood. 78:55.
Simmons and Torok-Storb (1991b) Blood. 78:2848.
Tavassoli and Friedenstein (1983) Ann J Hematol. 15:195.
Tavassoli and Crosby (1968) Science. 161:54.
Testa et al. (1988) Long-term Bone Marrow Damage after Cytotoxic Treatment:Stem Cells and Microenvironment in Hematopoiesis: Long-term Effects of Chemotherapy and Radiation (Hematology/vol. 8), eds. Testa, N.G., Gale, R.P., Marcel Dekker, Inc. New York and Basel, 1988, pp. 75-92.
Van Vlasselaer et al (1994) Blood. 84:753.
Waller et al. (1995) Blood. 85:2422.
Weiss (1976) Anatomical Record. 186:161.
Clarke, Emer, “Mesenchymal Cells” www.stemcell.com (mini-review).
Hellstrom et al. (1999) Development 126, 2047.
Zoltowska, et al. (2001) Arch Immunol Ther Exp (Warsz) 49:59-61.

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