Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Reexamination Certificate
2005-08-23
2005-08-23
Wax, Robert A. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
C435S207000, C435S069100, C424S486000, C424S078080
Reexamination Certificate
active
06933126
ABSTRACT:
Intracellular enzyme-activated fluorescent substrates that can be transported into a cell are provided. The membrane transportable fluorescent substrates are complexes (e.g., ionic complexes) formed between an enzyme activated fluorescent substrate and a carrier molecule. The fluorescent substrates can be used in an intracellular assay of enzyme activity and/or expression.
REFERENCES:
patent: 4507466 (1985-03-01), Tomalia et al.
patent: 4558120 (1985-12-01), Tomalia et al.
patent: 4568737 (1986-02-01), Tomalia et al.
patent: 4587329 (1986-05-01), Tomalia et al.
patent: 4631337 (1986-12-01), Tomalia et al.
patent: 4694064 (1987-09-01), Tomalia et al.
patent: 4713975 (1987-12-01), Tomalia et al.
patent: 4737550 (1988-04-01), Tomalia
patent: 4857599 (1989-08-01), Tomalia et al.
patent: 4871779 (1989-10-01), Killat et al.
patent: 5338532 (1994-08-01), Tomalia et al.
patent: 5527524 (1996-06-01), Tomalia et al.
patent: 5714166 (1998-02-01), Tomalia et al.
patent: WO 84/02705 (1984-07-01), None
Nolan et al., “Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction ofE. colilacZ,” (Proc Natl Acad Sci USA 85:2603-2607, 1988).
Westmark et al., “Selective monosaccharide transport through lipid bilayers using boronic acid carrieris,” J Am Chem Soc 118:11093-11100, 1996.
Morin et al., “Transport of glycosides through lipid organic membranes mediated by reversible boronate formation is a diffusion-controlled process,” J Am Chem Soc116:8895-8901, 1994.
Stephens, et al., “The Many Ways to Cross the Plasma Membrane”, Proc. Nat. Acad. Sci., 98, 8, 4295-4298 (2001).
Haensler, et al., “Polyamidoamine Cascade Polymers Mediate Efficient Transfection Cells in Culture”, Bioconjugate Chem., 4, 372-379 (1993).
Boussif, et al., “A Versatile Vector for Gene and Oligonucleotide Transfer Into Cells in Culture and in vivo: Polyethylenimine”, Proc. Nat. Acad. Sci. USA, 92, 7297-7301 (1995).
Kabanov, et al., “DNA Complexes with Polycations for the Delivery of Genetic Material Into Cells”, Bioconjugate Chem., 6, 7-20 (1995).
Tang, et al., “In Vitro Gene Delivery by Degraded Polyamidoamine Dendrimers”, Bioconjugate Chem., 7, 703-714 (1996).
Kukowska-Latallo, et al., “Efficient Transfer of Genetic Material Into Mammalian Cells Using Starburst Polyamidoamine Dendrimers”, Proc. Natl. Acad. Sci. USA, 93, 4897-4902 (1996).
Ozaki, et al., “Intracellular Delivery of Phosphoinositides and Inositol Phosphates Using Polyamine Carrires”, Proc. Nat. Acad. Sci., 97, 11286-11291 (2000).
Boon, et al., “Facilitated Phosphatidylcholine Flip-Flop Across Erthrocyte Membranes Using Low Molecular Weight Synthetic Translocases”, J. AM. Chem. Soc., 123, 6221-6226 (2001).
Wang, et al., “A Novel Biodegradable Gene Carrier Based on Polyphosphoester”, J. Am. Chem. Soc., 123, 9480-9481 (2001).
Morin, et al., “Transport of Glycosides Through Liquid Organic Membranes Mediated by Reversible Boronate Formation is a Diffusion-Controlled Process”, J. Am. Chem. Soc., 116, 8895-8901 (1994).
Westmark, et al., “Selective Monosaccharide Transport Through Lipid Bilayers Using Boronic Acid Carriers”, J. Am. Chem. Soc., 118, 11093-11100 (1996).
Applera Corporation
Kelber Steven B.
Kosson Rosanne
Merchant & Gould
Wax Robert A.
LandOfFree
Membrane transportable fluorescent substrates does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Membrane transportable fluorescent substrates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Membrane transportable fluorescent substrates will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3451375