Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-07-16
2000-02-15
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435325, 530323, 536 231, 536 245, C07H 2104, C12Q 168, C12N 1585
Patent
active
060251409
ABSTRACT:
Constructs of peptides and nucleic acid analogs conjugated together for transport across a lipid membrane and for delivery into interactive contact with intracellular polynucleotides are disclosed. Transport is effected through at least the exterior membrane of a cell, and most likely also through the walls of subcellular structures separated from the cytosol by lipid membranes, including the nucleus, mitochondria, ribosomes, etc. Peptide nucleic acid (PNA) analog sequences conjugated through a labile disulfide bond to transporting peptides, are intracellulary cleaved, and target mRNA (antigene) or dsDNA (antisense).
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Vinayak et al., "Automated Chemical Synthesis of PNA and PNA-DNA Chimera on a Nucleic Acid Synthesizer," Nucleosides & Nucleotides 16(7-9):1653-1656 (1997).
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Bartfai Tamas
Hallbrink Mattias
Langel Ulo
Pooga Margus
Saar Kulliki
Andrus Alex
Brusca John S.
PerSeptive Biosystems Inc.
Wang Andrew
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