Membrane holder

Details Membrane holder Membrane holder

1995-07-24

1997-01-07

Beisner, William

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

4352879, 4352972, 422101, 73 38, 73 6447, 324450, 324 711, C12M 112, C12M 134

Patent

active

055916368

ABSTRACT:
The invention provides a compact device of simple construction for holding a membrane in contact with a pair of fluids, one on each side of the membrane. According to the invention, the membrane holder comprises a base and an upper cap, with the membrane held between them. Fluids may be circulated over the surfaces of the membrane. Electrode ports are provided through which electrodes may be placed in proximity to the membrane for measuring or applying electrical potential across the membrane. The membrane holder comprises a base, which includes a lower plate and a center plate secured thereto, and an upper cap, which screws into the base by means of a threaded connection between them. The upper cap may be sealed to provide an enclosed volume for holding one of the fluids or the upper cap may be open to provide an open reservoir for holding the fluid. One or more membrane holders according to the invention may be placed in and held by a specially constructed heater stand. The heater stand includes internal channels for circulating a temperature controlled fluid through the heater stand to transfer heat to or from the base of the membrane holder.

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Product Brochure "Diffusion chamber system for cells cultured on Costar Snapwell.TM. microporous membranes," Precision Instruments Design. 1994.

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