Membrane fractions of 1,2- sn -diacylglycerol-enriched cells

Details Membrane fractions of 1,2- sn -diacylglycerol-enriched cells Membrane fractions of 1,2- sn -diacylglycerol-enriched cells

2001-10-26

2008-10-14

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Miscellaneous

C435S004000, C435S007200, C435S183000, C435S069100, C800S278000, C536S023200, C536S023600

Reexamination Certificate

active

07435583

ABSTRACT:
The invention concerns membrane fractions of cells containing a recombinant MGDG synthase and enriched with 1,2-sn-diacylglycerol, their preparation method, their use for screening molecules inducing MGDG synthase activity and a method for screening molecules inducing MGDG synthase activity using said membrane fractions.

REFERENCES:
patent: 2 790 915 (2000-09-01), None
Hawi et al. Detection of membrane-bound enzymes in cells using immunoassay and Raman microspectroscopy, Anal Biochem. Jun. 1, 1998;259(2):212-7.
Dorne et al., “Do Thylakoids Really Contain Phosphatidylcholine?” Proceedings of the National Academy of Sciences of the United States, vol. 87, No. 1, 1990, pp. 71-74.
Miège et al., “1,2-sn-Diacylglycerol in Plant Cells: Product, Substrate and Regulator,” Plant Physiology and Biochemistry (Paris), vol. 37, No. 11, Nov. 1999, pp. 795-808.
Miège, C., “Caractérisation Moléculaire et Biochimique de la Monogalactosyl Diacylglycerol Synthase (MGDG Synthase) de l'enveloppe des chloroplastes d'épinard,” Feb. 19, 1998, Université Joseph Fourier, Grenoble XP002178791, pp. 130.
Maréchal et al., “Kinetic Properties of Monogalactosyldiacylglycerol Snythase From Spinach Chloroplast Envelope Membranes,” Journal of Biological Chemistry, American Society of Biological Chemists , vol. 269, No. 8, Feb. 25, 1994, pp. 5788-5798.
Shimojima et al., “Cloning of the Gene for Monogalactosyldiacylglycerol Synthase and its Evolutionary Origin,” Proceedings of the National Academy of Sciences of USA, National Academy of Science, vol. 94, Jan. 1997, pp. 333-337.

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