Membrane filter agar medium containing two enzyme substrates use

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 4, 435 34, 435 38, 436172, C12Q 102, C12Q 100, C12Q 104, C12Q 110

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060635908

ABSTRACT:
An improved method for detection of total coliforms and E. coli comprising placing the target sample in a broth containing an ingredient that will encourage growth and repair of injured coliforms, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.

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