Membrane-bound cytokine compositions comprising GM=CSF and metho

Details Membrane-bound cytokine compositions comprising GM=CSF and metho Membrane-bound cytokine compositions comprising GM=CSF and metho

1997-07-29

1999-04-06

Spector, Lorraine

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424 932, 4241921, 424 851, 435 697, 435325, 4352523, 530351, 536 234, A61K 4800

Patent

active

058914323

ABSTRACT:
The present invention provides a cellular vaccine having a membrane-bound fusion protein that includes a non-antibody immunomodulatory molecule such as GM-CSF operatively fused to a heterologous membrane attachment domain. Non-antibody immunomodulatory molecules useful in the invention include immunostimulatory and immunosuppressive molecules such as cytokines. In one embodiment, the invention provides a cellular vaccine having a membrane-bound fusion protein that includes a non-antibody immunomodulatory molecule operatively fused to a heterologous membrane attachment domain and, additionally, a disease-associated antigen or immunogenic epitope thereof. Further provided by the invention are methods of modulating an immune response against a disease-associated antigen by administering to an individual a cellular vaccine having a membrane-bound fusion protein that includes a non-antibody immunomodulatory molecule operatively fused to a heterologous membrane attachment domain.

REFERENCES:
patent: 5109113 (1992-04-01), Caras et al.
patent: 5616477 (1997-04-01), Price
patent: 5637483 (1997-06-01), Dranoff et al.
patent: 5662907 (1997-09-01), Kubo et al.
patent: 5759535 (1998-06-01), Cohen
H. Bueler et al., Molecular Medicine 2(5):545-555, Sep. 1996.
Belardelli, "Role of Interferons and Other Cytokines in the Regulation of the Immune Response," APMIS 105:161-179 (1995).
Blachere and Srivastava, "Heat Shock Protein-Based Cancer Vaccines and Related Thoughts on Immunogenicity of Human Tumors," Seminars in Cancer Biology 6:349-355 (1995).
Dranoff et al., "Vaccination With Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity," Proc. Natl. Acad. Sci., USA 90:3539-3543 (1993).
Francisco and Georgiou, "The Expression of Recombinant Proteins on the External Surface of Escherichia coli, " Ann NY Acad Sci. 745:372-382 (1994).
Fan et al., "The Proinflammatory Cytokine Interleukin-12 Occurs as a Cell Membrane-Bound Form on Macrophages," Biochem. Biophys. Research Comm. 225:1063-1067 (1996).
Hakim et al., "A Nine-Amino Acid Peptide From IL-1.beta. Augments Antitumor Immune Responses Induced by Protein and DNA Vaccines," The Journal of Immunology 157:5503-5511 (1996).
Jadus et al., "Macrophages Can Recogize and Kill Tumor Cells Bearing the Membrane Isoform of Macrophage Colony-Stimulating Factor," Blood 87:5232-5241 (1996).
Lukacs et al., "Tumor Cells Transfected with a Bacterial Heat-Shock Gene Lose Tumorigenicity and Induce Protection Against Tumors," J. Exp. Med. 178:343-348 (1993).
Murphy and vanderSpek, "Targeting Diphtheria Toxin to Growth Factor Receptors," Seminars in Cancer Biology 6:259-267 (1995).
Musiani et al., "Cytokines, Tumour-Cell Death and Immunogenicity: A Question of Choice," Immunology Today 18:27-31 (1997).
Ni and O'Neill, "The Role of Dendritic Cell in T Cell Activation," Immunology and Cell Biology 75:223-230 (1997).
Pardoll, "Paracrine Cytokine Adjuvants in Cancer Immunotherapy," Ann. Rev. Immunol. 13:399-415 (1995).
Perez et al., "A Nonsecretable Cell Surface Mutant of Tumor Necrosis Factor (TNF) Kills by Cell-to-Cell Contact," Cell 63:251-258 (1990).
Robbins and Kawakami, "Human Tumor Antigens Recognized by T Cells," Curr. Opin. in Immunology 8:628-636 (1996).
Sasaki et al., "Cell-to-Cell Interaction of Cytokine-Dependent Myeloblastic Line Constitutively Expressing Membrane-Bound Stem Cell Factor Abrogates Cytokine Dependency Partially Through Granulocyte-Macrophage Colony-Stimulating Factor Production," Blood 85:1220-1228 (1995).
Schmidt-Wolf and Schmidt-Wolf, "Cytokines and Gene Therapy," Immunology Today 16:173-175 (1995).
Silva et al., "Characterization of T Cells that Confer a High Degree of Protective Immunity Against Tuberculosis in Mice After Vaccination With Tumor Cells Expressing Mycobacterial hsp65," Infection and Immunity 64:2400-2407 (1996).
Steinman, "Dendritic Cells and Immune-Based Therapies," Exper. Hematol. 24:859-862 (1996).
Tao and Levy, "Idiotype/Granulocyte-Macrophage Colony Stimulating Factor Fusion Protein as a Vaccine for B-cell Lymphoma," Nature 362:755-758 (1993).
Tepper and Mule , "Experimental and Clinical Studies of Cytokine Gene-Modified Tumor Cells," Human Gene Therapy 5:153-164 (1994).

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