Membrane based dot immunoassay and method of use

Details Membrane based dot immunoassay and method of use Membrane based dot immunoassay and method of use

1991-12-30

1993-04-06

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 72, 435 794, 435 732, 435970, 436531, 436810, 422 57, 427 2, C12Q 170, G01N 33545

Patent

active

052003124

ABSTRACT:
Antigens or antibodies are detected using a novel membrane based immunoassay. Known antigens or antibodies which will form complexes with antigens/antibodies to be assayed are spot filtered with pressure through a membrane. The membrane, either by itself or attached to a base material as a test strip, is incubated with a test fluid. Consequently, the resulting antibody-antigen complex is incubated directly or after an intermediate anti-antibody incubation with enzyme conjugated immunoglobulin and exposed to substrate which produces a colored insoluble product if the test target is present.

REFERENCES:
patent: 4391904 (1983-07-01), Litman et al.
patent: 4774177 (1988-09-01), Marks
patent: 4797259 (1989-01-01), Matkovich et al.
Oprandy et al., Am. J. Trop. Med. Hyg. vol. 38, No. 1, pp. 87-108, (1988).
Oprandy et al., J. Clin. Micb., vol. 27, No. 1, pp. 74-77, (1989).
Porter et al; Nucleic Acids Research, vol. 19, No. 14, 1991, p. 4011.
Program and Abstracts of the 40th Annual Meeting of the American Society of ropical Medicine and Hygiene; vol. 45, No. 3, Sep. 1991.
Oprandy and Long; Journal of Clinical Microbiology, vol. 28, No. 8, Aug. 1990, pp. 1701-1703.

Affiliated with

Also associated with

Rating

Membrane based dot immunoassay and method of use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Membrane based dot immunoassay and method of use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Membrane based dot immunoassay and method of use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-535789