Membrane assay using focused sample application

Details Membrane assay using focused sample application Membrane assay using focused sample application

1987-12-03

1989-04-04

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

422 56, 422 58, 435 5, 435 6, 435 7, 435805, 435810, 436810, 436813, G01N 3353, G01N 33569

Patent

active

048186775

ABSTRACT:
Methods and kits are described for performing immunoassays. The kit includes a reaction cell having a microporous membrane and an absorbent capable of drawing liquid sample through the membrane. An applicator is provided for applying a small volume of the liquid sample to the membrane by contacting a port of the applicator to the membrane and allowing the sample to flow therethrough by capillary action. Analyte in the sample is immobilized, typically by immunoadsorption, and the immobilized analyte may then be visualized using conventional signal producing systems, such as color, fluorescence, and luminescent systems.

REFERENCES:
patent: 3888629 (1975-06-01), Bagshawe
patent: 4366241 (1982-12-01), Tom et al.
patent: 4631174 (1986-12-01), Kondo
patent: 4632901 (1986-12-01), Valkirs et al.
Package Insert-TestPack.RTM., Abbott Laboratories, North Chicago, Ill. 60064, 2 pages.

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