Membrane affinity concentration immunoassay

Details Membrane affinity concentration immunoassay Membrane affinity concentration immunoassay

1991-02-28

1993-04-27

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436539, 436810, 435 5, 435 71, 435 792, 435 793, 435 794, 435 795, 435180, 435971, G01N 33538, C12Q 100

Patent

active

052061787

ABSTRACT:
Methods for determining the presence and/or concentration of an analyte in a biological fluid sample are disclosed. The methods generally include admixing in solution certain polymer/reactant and reporter/reactant conjugates along with the biological fluid sample suspected of containing the analyte, thereby forming ternary complexes. The separation of the complexes from the reaction mixture is achieved through the affinity of certain selected polymer compositions for various solid phases. Upon separation, the amount of reporter activity in the solution may be measured, and therefrom the presence and/or concentration of the analyte determined. Multiple analyses on a biological fluid sample suspected of containing one or more analytes may also be performed, using either a variety of different reporters or selected polymers having varied affinity for the solid phase.

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