Megakaryocyte stimulatory factor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530395, 530830, 530835, 530412, 530417, 530413, 435 695, 435 696, 424 851, C07K 1500

Patent

active

051552116

ABSTRACT:
A megakaryocyte stimulatory factor (MSF), purified to homogeneity, is an acidic protein (pI=5.1) with an Mr=15,000 which stimulates PF4-like protein synthesis in rat promegakaryoblast cells by as much as 7-fold, and exhibits half-maximal activity at a concentration of 0.8 pM. MSF exhibits no biologic activity corresponding to other known hemopoietic growth factors, and appears to be specific for the megakaryocyte lineage.
In the given examples, MSF was purified to homogeneity (as judged by SDS-PAGE and isoelectric focusing in the presence of 9.2 M urea) from serum-free conditioned medium obtained from cultured human embryonic kidney (HEK) cells, and to near homogeneity from thrombocytopenic plasma. The MSF is isolated by precipitating the MSF with ammonium sulfate at 80% saturation, removing insoluble, non-MSF material and applying the soluble protein to a WGA-Sepharose column, eluting the MSF protein with chitin oligosaccharides, applying the concentrated eluant containing MSF activity to a Biogel P200 column, and chromatographing the eluted MSF fractions on a TSK-G3000 HPLC size exclusion column.

REFERENCES:
T. P. McDonald, et al., Exp. Hematol., 9:288-296 (1981).
J. Levin, et al., Blood, 54:166a (abstr.) (1979).
T. P. McDonald and C. Nolan, Biochem. Med., 21:146-155 (1979).
P. Cohen, et al., Journal of Clinical Investigation, 44:1036 (abstr.) (1965).
P. T. Jubinsky and E. R. Stanley, Proceedings of the National Academy of Science U.S.A., 82:2764-2768 (1985).
T. P. McDonald, et al., J. Lab. Clin. Med., 85:59-66 (1975).
S. M. Greenberg et al., J. of Biol. Chem., 262:3269-3277 (1987).
G. W. Bazill et al., Biochem. J., 210:747-759 (1983).
J. Levin et al., Blood, 60(4):989-988 (1982).
G. Tayrien et al., J. of Biol. Chem.,262(7):3262-3268 (1987).
R. M. Leven et al., Blood, 69(4):1046-1052 (1987).
Shaneva et al., Experimental Hematology 15, 1987, pp. 657-63.
Di Alarcon, Blood Cells, 15, 1989, pp. 173-185.
Di Alarcon, Blood Cells, 15, 1989, pp. 186-191.
Williams et al., Megakeryoryte Biology, and Precursors, 1981, pp. 59-75.
Kuriya et al., Blood Cells, 12, 1986, pp. 233-247.
Hoffman et al., Blood Cells, 13, 1987, pp. 73-85.
Wong, Blood Cells, 15, 1989, pp. 205-229.
Bagnaru et al., Exp. Hematol, 15, 1987, pp. 679-684.
Chaudhury et al., J. Lab. Clin Med., 1989, vol. 114, pp. 382-388.
Prog Clin. Biol. Res. 215, 1986, ed. Stranenu, pp. 201-208.
Yang et al., CA vol. 105(5) 1986, #36292X.
Hoffman et al., N.E. J. Med. 305, 1981, pp. 533-538.
Hoffman et al., J. Clin. Invest. 75, 1985, pp. 1174-1182.
McDonald et al., Exp. Hematol. 10(6) 1982, pp. 544-550.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Megakaryocyte stimulatory factor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Megakaryocyte stimulatory factor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Megakaryocyte stimulatory factor will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1301622

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.