Medium for the separation of human blood leucocytes

Liquid purification or separation – Processes – Making an insoluble substance or accreting suspended...

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210927, 210789, 210731, B01D 2101, B01D 2126

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active

042552567

ABSTRACT:
A rapid one-step density separation method for separating and purifying whole blood into mononuclear and polymorphonuclear leucocyte and erythrocyte fractions wherein use is made of a separating medium consisting of a single aqueous solution of a high molecular weight sucrose or glucose polymer, and a water soluble metrizoate or diatrizoate compound, the density of the solution being greater than 1.095 gm/ml at room temperature and the concentration of the sucrose or glucose polymer lying in the range of 5 to 10% (based on weight/total volume of solution).

REFERENCES:
patent: 3291693 (1966-12-01), Brown
patent: 3513976 (1970-05-01), James
patent: 3519400 (1970-07-01), Anderson
patent: 3814687 (1974-06-01), Ellis et al.
patent: 4027660 (1977-06-01), Wardlaw et al.
patent: 4083784 (1978-04-01), Zine, Jr.
patent: 4190535 (1980-02-01), Luderer et al.

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