Medium for preserving tissue without tissue culturing occurring

Details Medium for preserving tissue without tissue culturing occurring Medium for preserving tissue without tissue culturing occurring

1997-10-08

1999-07-20

Yu, Mickey

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

A01N 102

Patent

active

059255102

ABSTRACT:
A method for autotransplantation, is provided including the steps of (a) obtaining a piece of tissue from one part of a body, (b) preserving the tissue by contacting it with a tissue preserving medium, without tissue culturing occurring, and (c) grafting the tissue in another part of the body. The method is especially advantageous for the repair of hypospadias using buccal mucosa tissue. The tissue preserving medium preferably is a solution of (a) 80-120 mM NaCl, (b) 3-6 mM KCl, (c) 0.1-0.3 mM MgSO.sub.4.7H.sub.2 O, (d) 1-3 mM CaCl.sub.2.2H.sub.2 O, (e) 0.2-0.6 mM NaH.sub.2 PO.sub.4, (f) 1.5-4 mM glucose, (g) 15-25 mM Na lactate, (h) 0.2-0.6 mM Na pyruvate, (i) 0.01-0.05 mM phenol red, (j) 0.1-0.4 mM L-glutamine, (k) 2-35 mM sodium bicarbonate, (l) 125-200 units/ml penicillin-G, (m) 40-60 .mu.g/ml streptomycin sulfate, (n) vehicle consistent with tissue preservation; said medium having a pH ranging from 7.0-7.8.

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