Medium for plant protoplast culture

Details Medium for plant protoplast culture Medium for plant protoplast culture

1983-08-25

1985-08-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435241, 435948, 47 58, A01B 7900, A01C 100, C12N 500, C12N 502

Patent

active

045336369

ABSTRACT:
A culture medium and method for the isolation and culture of plant protoplasts is disclosed which includes in it a quantity of neutral mammalian blood serum. An addition of 1 to 25% serum to protoplast media results in a greater yield of viable protoplast cells, helps to protect the protoplasts in culture, and makes it possible to obtain and maintain corn protoplasts, a heretofore difficult task.

REFERENCES:
Kassanis et al., Biol. Abst., vol. 67, (10), No. 63312, "Inhibition of Tobacco Mosaic Virus Multiplication in Tobacco Protoplasts by Rabbit Serum".

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