Medium for ES culturing

Details Medium for ES culturing Medium for ES culturing

2011-04-12

2011-04-12

Lankford, Leon B (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S325000, C435S366000, C435S383000, C435S395000, C435S404000, C435S405000, C435S406000, C435S407000

Reexamination Certificate

active

07923245

ABSTRACT:
The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a basal medium for producing the medium thus described. The basal medium of the present invention is characterized by that it has composition shown by Table I. Further, the present invention discloses a medium for ES cells produced with the basal medium.

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International Search Report for PCT/JP2004/019818 completed Feb. 8, 2005.
Supplementary Search Report for EP 04 80 8168.
Johansson et al., “Evidence for Involvement of Actvin A and Bone Morphogenetic Protein 4 in Mammalian Mesoderm and Hematopoietic Development,”Molecular and Cellular Biology, Jan. 1995, pp. 141-151, vol. 15(1).

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