Medium for enhanced staining of single strand nucleic acids...

Details Medium for enhanced staining of single strand nucleic acids... Medium for enhanced staining of single strand nucleic acids...

2011-01-18

2011-01-18

Neckel, Alexa D (Department: 1795)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S606000, C204S461000, C204S616000

Reexamination Certificate

active

07871507

ABSTRACT:
A medium is provided, being adapted to be applied in electrophoretic separation of nucleic acids. The medium comprises a staining reagent adapted to stain nucleic acids. Said medium further comprises a urea derivative, adapted to interact with said staining reagent and thereby providing an enhanced staining of said nucleic acids. Further, an electrophoresis device is provided, adapted to perform electrophoretic separation of nucleic acids, comprising the before medium and comprising electrodes for applying an electrical field across the medium. A method to perform electrophoresis using the before electrophoresis device and medium is provided.

REFERENCES:
patent: 4950708 (1990-08-01), Hochstrasser
patent: 6938476 (2005-09-01), Chesk
patent: 2004/0204523 (2004-10-01), Kratzmeier
patent: 2006/0057566 (2006-03-01), Van Ness et al.
Althaus et al. (“Two-dimensional gel electrophoresis of myelin and oligodendroglial proteins solubilized by a mixture of tetramethylurea and dimethylethyleneurea”, Electrophoresis 1983, 4, 347-353).
Barker et al. (“Ureas as Solvents for Chemical Investigations”, Angew. Chem. Inr. Ed. Engl. 18, 503-507, 1979).
Khurgin et al. (“Investigation of Intermolecular Interactions in Solutions By Means of Millimeter-Wave Spectroscopy”,1990 Plenum Publishing Corporation, pp. 265-270).
Product Information of Dimeric Cyanine Nucleic Acid Stains, Jan. 2000 (“Product Information”).

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