Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1982-03-29
1984-08-28
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 435253, 435885, C12Q 114, C12Q 104, C12N 120, C12R 146
Patent
active
044684568
ABSTRACT:
A medium for the selective growth and identification of Streptococcus mutans bacteria is disclosed, that includes a tryptone-glucose extract agar, monobasic and dibasic potassium phosphate, yeast extract, agar, a color indicator for the bacteria and a solution of sucrose ranging in concentration from 1% to 15%. Preferably, the concentration of the sucrose may range from about 3% to about 11%. A bacteria culture plate may be prepared comprising the medium, and may include a first basal layer comprised entirely of the medium, and a second overlayer agar coating, including a mixture of the medium with a calcium phosphate suspension. Both the basal layer and the overlayer agar coating are preferably adjusted to a mildly basic pH.
The present medium, and bacteria culture plates prepared therewith, offer desired bacterial specificity with no growth inhibition, that is usually the case with specific media of this kind. The present medium and bacteria culture plate is particularly useful, as Streptococcus mutans is considered to be instrumental in dental caries development.
REFERENCES:
patent: 3890200 (1975-06-01), Jordan et al.
patent: 3935067 (1976-01-01), Thayer
patent: 4102743 (1978-07-01), Yokobayashi et al.
patent: 4397944 (1983-08-01), Komura et al.
Ducker et al., "Comparative Effects of the Substance-Sweetners Glucose, Sorbitol, Sucrose, Xylitol, and Trichlosucrose", Arch. Oral Biol. 24(12) (1979), pp. 965-970, Chem. Abst. 93: 89294.
Nakamura et al., "New Caries Activity I in Vitro Test of the Test Solution S-3105", Koku Eisei Gakkai Zasshi 30(4), 1980, pp. 376-381, Chem. Abst. 94: 135357h.
Emilson et al., "Growth of Streptococcus mutans on Various Selective Media", Journal of Clinical Medicine 4(1), (1976), pp. 95-98, Abst.
Sudo et al., "Model System for Studying Colonization and Growth of Bacteria on a Hydroxyapatite Surface", Infection and Immunity 12(3), (1975), pp. 576-585.
Otake et al., "Virulence of Streptococcus mutans: Characterization of a Serotype g Antigen Defective Mutant and its Revertants", Infection and Immunity 31(1), (1980), pp. 151-159.
Skinner et al., "Influence of Sugar Type on the Pattern of Acid Production by Streptococcus mutans", Journal of Dental Research 51(4), (1972), pp. 1022-1024.
Nath Gary M.
Person Sandra M.
Tarcza John E.
Warner-Lambert & Company
Wiseman Thomas G.
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