Medium for culturing normal human epidermal melanocytes

Details Medium for culturing normal human epidermal melanocytes Medium for culturing normal human epidermal melanocytes

1997-03-20

1999-06-29

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

435406, 435407, 435408, C12N 500

Patent

active

059168099

ABSTRACT:
Provided is a medium for culturing normal human epidermal melanocytes in vitro. The medium comprises a basal medium for culturing animal cells, Ca.sup.2+ at a final concentration of between about 0.15 mM and about 1.2 mM and Mg.sup.2+ at a final concentration of 1.2 mM and 6 mM or Ca.sup.2+ at a final concentration of between about 0.9 mM and about 1.2 mM and Mg.sup.2+ at a final concentration between about 0.6 mM and 6.0 mM and 0.001 to 0.1% scrum (v/v). The medium can promote both dendrite formation and proliferation of the cells.

REFERENCES:
patent: 4673649 (1987-06-01), Boyce et al.
Tennebaum et al., "Magnesium and Phosphate Enrichment of Culture Medium Stmulates the Proliferation of Epidermal Cells from Newborn and Adult Mice", J. Cell. Physiol. 143(3):431-8 (1990).
Medrano et al., "Successful Culture of Adult Human Melanocytes Obtained from Normal and Vitiligo Donors", J. Invest. Dermatol. 95(4):441-445 (1990).
Hara et al., "Role of Integrins in Melanocyte Attachment and Dendricity" J. Cell Sci. 107(10):2739-48 (1994).
GIBCO Catalog, p. 117 (1992).
Pittelkow et al., "Serum-Free Culture Of Normal Human Melanocytes: Growth Kinetics and Growth Factor Requirements", Journal of Cellular Physiology, vol. 140:565-576, (1980).
Boyce et al., "Cultivation, Frozen Storage, and Clonal Growth Of Normal Human Epidermal Keratinocytes In Serum-Free Media", Journal of Tissue Culture Methods, vol. 9(2):83-93, (1985).
Boyce et al., "Calcium-Regulated Differentiation Of Normal Human Epidermal Keratinocytes In Chemically Defined Clonal Culture and Serum-Free Serial Culture", The Journal of Investigative Dermatology, vol. 81(1):33s-40s, (1983).
Asami et al., "Identification of Trypsin Inhibitor in Bovine Pituitary Extracts As A Survival Factor For Adult Rat Hepatocytes In Primary Culture", J. Biochem., vol. 95(2):299-309, (1984).

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