Medium containing pipecholic acid and gamma amino butyric...

Details Medium containing pipecholic acid and gamma amino butyric... Medium containing pipecholic acid and gamma amino butyric...

2005-09-08

2008-11-11

Crouch, Deborah (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S366000

Reexamination Certificate

active

07449334

ABSTRACT:
Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.

REFERENCES:
patent: 2006/0014279 (2006-01-01), Xu et al.
patent: WO 2004/055155 (2004-07-01), None
Xu et al. Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells. Nature Biotechnology. vol. 19, pp. 971-974.
Miyazono, Kohei, “Positive and negative regulation of TGF-B signaling,” Journal of Cell Science 113:1101-1109 (2000).
Reya, T., et al., “A role for Wnt signalling in self-renewal of haematopoietic stem cells,” Natur 423:409-414 (2003).
Ying, Q., et al., “BMP Induction of Id Proteins Suppresses Differentiation and Sustains Embryonic Stem Cell Self-Renewal in Collaboration with STAT3,” Cell 115:281-292 (2003).
Amit, M., et al., “Feeder Layer- and Serum-Free Culture of Human Embryonic Stem Cells,” Biology of Reproduction 70:837-845 (2004).

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