Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-06-05
1998-07-14
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 38, 435802, 435968, C12Q 104, C12Q 110
Patent
active
057802599
ABSTRACT:
The invention relates to a target microbe-specific medium for detecting the presence or absence of a target microbe in an environmental or biological sample, the medium including: a) an effective amount of vitamin, amino acid, element and salt ingredients operable to allow viability and log phase reproduction of the target microbe in the presence of a nutrient-indicator and to aid the target microbe through lag phase and into log phase of growth in the sample; and b) an effective amount of a nutrient-indicator which is provided in an amount sufficient to support log phase growth of the target microbe of a sample until a detectable characteristic signal is produced in the medium/sample mixture during the log phase growth; the nutrient-indicator being incapable of supporting continued logarithmic growth of any viable non-target microbes in the medium/sample mixture to produce a detectable characteristic signal; and the nutrient-indicator being operable to alter a detectable characteristic of the medium/sample mixture if metabolized by the target microbe so as to confirm the presence or absence of the target microbe in the sample; wherein the medium lacks a gelling agent so that when the medium is mixed with a liquefied sample a liquid is formed, and wherein the ingredients in (a), and the nutrient-indicator are chosen such that growth of non-target microbes do not interfere with growth of the target microbe. The invention also relates to methods of detecting the presence or absence of a target microbe in which an environmental or liquid sample is mixed with the medium, and the mixture is then evaluated for the alteration of a detectable characteristic.
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