Medium and method for blotting macromolecules

Details Medium and method for blotting macromolecules Medium and method for blotting macromolecules

1994-03-23

1996-12-17

Woodward, Michael P.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436514, 436530, 436543, 435 792, 435 79, G01N 33543, G01N 33558, G01N 33544, G01N 3353

Patent

active

055852769

ABSTRACT:
A medium and method for blotting macromolecules is disclosed. The method begins with the separation of macromolecules on a slab gel. Next, a sheet of cellulosic paper is dipped into an alcohol bath. The dipped paper is placed against the slab gel. The paper and the gel are exposed to a means for transferring the macromolecules from the gel onto the paper. In a particularly advantageous embodiment of the method, the paper is dipped into a methanol bath, the transferring means includes an electric field, and the paper is either xerographic paper or cotton bond paper. The present invention is also a medium for blotting macromolecules comprising a piece of cellulosic paper that has been dipped in alcohol.

REFERENCES:
Stott, D. I., J. Immunol Meth 119:153-187. (1989).
Minamide et al., Anal Biochem 190, 66-70 (1990).
Murthy et al., Anal Biochem 64:18-29 (1975).
Conn, et al., Outlines of Biochemistry 5/E, 244-245, John Wiley & Sons, New York (1987).
Freifelder, D., Molecular Biology 2d Ed., pp. 133-135, Jones & Bartlett, Boston (1987).
Montgomery and Fu, "Detection of Cellulose-Binding Proteins in Electrophoresis Gels by Filter Paper Affinity Blotting," Anal. Biochem., 174[1]:204-208 (1988).
Stott, D. I., J. Immunol. Meth. 117:153-187 (1989).

Affiliated with

Also associated with

Rating

Medium and method for blotting macromolecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Medium and method for blotting macromolecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Medium and method for blotting macromolecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1990670