Media for normal human muscle satellite cells

Details Media for normal human muscle satellite cells Media for normal human muscle satellite cells

1992-08-12

1994-06-28

Elliott, George C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524021, 4352403, 43524031, C12N 500, C12N 508, C12N 506

Patent

active

053246560

ABSTRACT:
An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, serum-free supplements for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin fraction mitogen, epidermal growth factor and insulin are described. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC, which can be transplanted to muscles of patients afflicted with muscle degenerative diseases such as muscular dystrophy. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC.

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