Media for normal human muscle satellite cells

Details Media for normal human muscle satellite cells Media for normal human muscle satellite cells

1988-11-01

1992-09-01

Doll, John

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524031, 4352403, C12N 508, C12N 500

Patent

active

051438429

ABSTRACT:
An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, a serum-free supplement for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin epidermal growth factor and insulin. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC.

REFERENCES:
patent: 4443546 (1984-04-01), Strmerman et al.
Blau, H. M. and C. Webster (1981) PNAS 78: 5623-5627.
Lim, R. W. and S. D. Hauschka (1984) Developmental Biology 105: 48-58.
Askanas, V. et al. (1985) Neurosci. Lett. 61: 213-219.
Linkhart, T. A. et al. (1982) Cold Spring Harbor Conf. on Cell Proliferation 9: 867-876.
Dollenmeier, P. et al. (1981) Exp. Cell Res. 135: 47-61.
Ham, R. G. (1963) Experimental Cell Research 29: 515-526.
Hauschka, S. D. and I. R. Konigsberg (1966) PNAS 55: 119-126.
Hauschka, S. D. (1974) Developmental Biology 37: 329-344.
Blau, H. M. et al. (1983) PNAS 80: 4856-4860.
Ham, et al., Media and Growth Requirements; in Methods in Enzymology, vol. LVIII, pp. 89-93, 1979.
Knedler, et al., Optimized Medium for Clonal Growth of Human Microvascular Endothelial Cells with Minimal Serum, In Vitro Cellular & Developmental Biol., vol. 23, No. 7, pp. 481-491, 1987.
Florini, et al., A Serum-Free Medium for the Growth of Muscle Cells in cultures In Vitro, vol. 15, No. 12, pp. 983-992, 1979.
Allen, et al., A Serum-Free Medium that Supports the Growth of Cultured Skeletal Muscle Satellite Cells, In Vitro Cellular & Developmental Biol., vol. 21, No. 11, pp. 636-640, 1985.
Ham, et al., Improved Media for Normal Human Muscle Satellite Cells, In Vitro Cell. Devel. Biol., vol. 24, No. 8, pp. 833-844, Aug., 1988.
Askanas, V. and Gallez-Hawkins, G. (1985a) Arch. Neurol. 42: 749-752.
Knedler, A. and Ham, R. G. (1987) In Vitro Cell. Develop. Biol. 23(7):481-491.
Askanas, V. et al. (1985b) Soc. Neurosci. Abstr. 11: 936.
Delaporte, C. et al. (1986) Biol. Cell. 57: 17-22.
Yasin, R. and van Beers, G. (1983) in Hormonally Defined Media: A Tool in Cell Biology, Fischer, and Weiser (eds.), Springer Verlag, Berlin, pp. 406-410.
Yasin, R. and van Beers, G. (1983) in Hormonally Defined Media: A Tool in Cell Biology, Fischer, and Weiser (eds.), Springer Verlag, Berlin, pp. 406-410.

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