Media concentrate technology

Details Media concentrate technology Media concentrate technology

1993-09-21

1995-12-12

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352402, 4352403, 435244, 252380, 252397, 252401, C12N 500, C12N 104

Patent

active

054749310

ABSTRACT:
The present invention relates to a method of subgrouping media formulations into stable, compatible components that can then be solubilized at high concentrations (10.times. to 100.times.). Concentrated solutions of the ingredient containing subgroups can be used to prepare media formulations. Concentrated culture media formulations (2-10.times.) or 1.times. cell culture media can be prepared according to the present invention by mixing a sufficient amount of the concentrated subgroup solutions with a sufficient amount of a diluent (water, buffer, etc.). The present invention further relates to stabilization of glutamine by complexing or mixing glutamine with divalent metal cations in solution.

REFERENCES:
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Bray et al., Biochem. J. 44:625-627 (1949).
Tritsch et al., Experimental Cell Research 28:360-364 (1962).
Wang et al., In Vitro 14(8):715-722 (1978).
Ozturk et al., Biotechnol. Prog. 6:121-128 (1990).
Griffin et al., Cancer Research 41:2241-2248 (1981).
Ham, R. G., Proc. N.A.S. 53:288-293 (1965).
Waymouth, C., Methods for Preparation of Media, Suppl., and Substrata for Serum-Free Animal Cell Culture, pp. 23-68 (1984).
Lidgate et al., BioPharm pp. 28-31 (1989).
Masson, G., Food Microstructure 8:11-14 (1989).

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