Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se
Patent
1995-05-19
1997-10-28
Czaja, Donald E.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Culture medium, per se
252380, 252397, 252401, 435431, C12N 500, C12N 104
Patent
active
056817487
ABSTRACT:
The present invention relates to a method of subgrouping media formulations into stable, compatible components that can then be solubilized at high concentrations (10.times. to 100.times.). Concentrated solutions of the ingredient containing subgroups can be used to prepare media formulations. Concentrated culture media formulations (2-10.times.) or 1.times. cell culture media can be prepared according to the present invention by mixing a sufficient amount of the concentrated subgroup solutions with a sufficient amount of a diluent (water, buffer, etc.).
The present invention further relates to stabilization of glutamine by complexing or mixing glutamine with divalent metal cations in solution.
REFERENCES:
patent: 4879222 (1989-11-01), Alderman et al.
patent: 5474931 (1995-12-01), DiSorbo
Ham, R. G., "An Improved Nutrient Solution for Diploid Chinese Hamster and Human Cell Lines", Experimental Cell Research (29):515-526 (1963).
Price and Evans, "Preparation of Medium NCTC 163", TCA Manual 3(1):497-501 (1976).
Moore and Woods, "Culture Media for Human Cells-RPMI 1603, RPMI 1634, RPMI 1640 and GEM 1717", TCA Manual 3(1):503-509 (1976).
Healy and Macmorine, "Chemically Defined Basal Medium CMRL-1969*", TCA Manual (3)1:511-515 (1976).
Waymouth, Charity, "Preparation of Medium MAB87/3 for Primary Cultures of Epithelial Cells", TCA Manual (3)1:521-525 (1976).
Huang and Murashige, "Plant Tissue Culture Media: Major Constituents, Their Preparation and Some Applications*", TCA Manual (3)1:539-548 (1976).
Leibovitz, Albert, "Preparation of Medium L-15", TCA Manual (3)2:557-559 (1977).
Morton and Tolnai, "Preparation of Medium 199", TCA Manual (4)1:729-736 (1978).
Kirk, David, "Serium-Free Cell Culture of Normal Human Urothelium", Journal of Tissue Culture Methods (9)2:37-41 (1984).
Bray et al., "The Enzymic Hydrolysis of Glutamine and its Spontaneous Decomposition in Buffer Solutions", Biochem. J. 44:625-627 (1949).
Griffin et al., "Kinetics of Phototoxicity of Fisher's Medium for L5178Y", Cancer Research 41:2241-2248 (1981).
Ham, R. G., "Clonal Growth of Mammalian Cells in a Chemically Defined, Synthetic Medium", Proc. Natl. Acad. Sci. USA 53:288-293 (1965).
Lidgate et al., "Using a Microfluidizer to Manufacture Parenteral Emulsions", BioPharm:28-31 (1989).
Masson, G., "Advanced Techniques for Preparation and Characterization of Small Unilamellar Vesicles", Food Microstructure 8:11-14 (1989).
Ozturk and Palsson, "Chemical Decomposition of Glutamine in Cell Culture Media, Effect of Media Type pH and Serum Concentration", Biotechnol. Prog. 6(2):121-128 (1990).
Sigma Cell culture reagents 1991 catalogue/price list (OPI-Sep. 25, 1990) pp. 249-251, 279-282.
Tewari et al., "Formation and Stabilities of Bivalent Metal Ion Chelates of L-Glutamine", Chemical Abstracts 79 (16): 373, Abstract No. 97604s (1973) and J. Inorg. Nucl. Chem. 35(7):2441-2446 (1973).
Tritsch and Moore, "Spontaneous Decomposition of Glutamine in Cell Culture Media", Experimental Cell Research 28:360-364 (1962).
Wang and Nixon, "Identification of Hydrogen Peroxide as a Photoproduct Toxic to Human Cells in Tissue-Culture Medium Irradiated with Daylight Fluroescent Light", In Vitro 14(8):715-722 (1978).
Waymouth, C., Methods for Preparation of Media, Supplements, and Substrata for Serum-Free Animal Cell Culture, ed. Alan R. Liss, Inc., NY, pp. 23-68 (1984).
DiSorbo Dennis M.
Jayme David
Czaja Donald E.
Koh Choon P.
Life Technologies Inc.
LandOfFree
Media concentrate technology does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Media concentrate technology, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Media concentrate technology will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1025673