Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Patent
1995-05-02
1996-12-24
Warden, Jill
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
436 92, 436 98, 436161, 436174, 436177, 436816, 436901, 73 6152, 73 6153, 73 6155, 210635, 210656, 210660, 2101982, G01N 118
Patent
active
055873235
ABSTRACT:
A improved method for preparing a concentrated meconium extract containing basic and acid-neutral target analytes present in a meconium sample suspected of containing the target analytes is provided. The meconium sample is extracted with a mixed solvent of acetonitrile and a volatile organic acid and the resulting organic phase is separated. The organic phase is diluted with water to provide an organic solution which is passed through a mixed mode solid phase extraction column having strong cation exchange and hydrophobic functionality. Acid-neutral and basic target analytes are sequentially eluted from the column and the eluates are combined to form a combined extract. The combined extract is evaporated to dryness and reconstituted in buffer to provide a clean concentrated neonatal meconium extract for use in qualitative and quantitative analyses.
REFERENCES:
patent: 4771005 (1988-09-01), Spiro
patent: 5015589 (1991-05-01), Ostrea, Jr.
patent: 5185267 (1993-02-01), Ostrea, Jr.
patent: 5326708 (1994-07-01), Lewis
patent: 5532131 (1996-07-01), Lewis
Browne et al. Journal of Chromatography-Biomedical Applications, vol. 575, 1992, pp. 158-161.
Murphey et al. Journal of Chromatography-Biomedical Applications, vol. 613, 1993, pp. 330-335.
Mahone et al. American Journal of Obstetrics & Gynecology, vol. 171, No. 2, Aug. 1994, pp. 465-469.
Lewis et al. Clinical Toxicology, vol. 32 (6), 1994, pp. 697-703.
Ostrea et al. The Journal of Pediatrics, vol. 122, No. 1, Jan. 1993, pp. 152-154.
Lewis Douglas E.
Moore Christine M.
United States Drug Testing Laboratories, Inc.
Wallenhorst Maureen M.
Warden Jill
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