Mechanically activated channel blocker

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

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Details

C530S300000, C530S324000, C514S002600

Reexamination Certificate

active

07125847

ABSTRACT:
The present invention discloses a peptide of SEQ ID NO:2 and its variants that blocks stretch-activated ion channels. The peptide, designated as GsMTx-4, is present in the venom of the spiderGrammostola spatulata. The present invention also discloses a method of purifying the peptide GsMTx-4 from the spider venom and a method for inhibition of stretch activated ion channels in a cell. The cDNA sequence encoding the GsMTx-4 is also disclosed. This peptide and its variants can be used for the treatment of cardiac arrhythmias.

REFERENCES:
patent: 4925664 (1990-05-01), Jackson et al.
patent: 5756663 (1998-05-01), Lampe et al.
patent: 5968838 (1999-10-01), Lampe et al.
Suchyna et al. Identification of a Peptide Toxin fromGrammastola spatulataSpider Venom that Blocks Cation-Selective Stretch-activated Channels. May 2000, J. Gen. Physiol. vol. 115, pp. 583-598.
Craik et al. The cystine knot motif in toxins and implications for drug design. 2001, Toxicon, vol. 39, pp. 43-60.
Nazir et al.,Effects of G. spatulata Venom, a Novel Stretch-Activated Channel Blocker in a Model of Stretch-Induced Ventricular Fibrillation in the Isolated Heart, Abstracts from the 68thScientific Sessions, Oct. 15, 1995, vol. 2 (Suppl. I), p. 641.

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