Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2004-09-22
2008-07-22
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C436S161000, C436S544000, C436S546000, C436S824000, C530S389800, C530S402000, C530S412000
Reexamination Certificate
active
07402398
ABSTRACT:
The invention provides methods and kits for detecting and/or measuring receptor homodimers on a cell surface membrane. In one aspect, the methods employ pairs of probes comprising binding compounds and a cleaving probe, such that at least one binding compound binds specifically to the same epitope of a membrane-bound analyte as the cleaving probe. The binding compound includes one or more molecular tags attached through a cleavable linkage, and the cleaving probe includes a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to a homodimer of a cell surface molecules results in release of molecular tags from the binding compounds, providing a measure of formation of the homodimeric complex.
REFERENCES:
patent: 4331590 (1982-05-01), Bocuslaski
patent: 4650750 (1987-03-01), Giese
patent: 4709016 (1987-11-01), Giese
patent: 4780421 (1988-10-01), Kameda
patent: 5340716 (1994-08-01), Ullman
patent: 5360819 (1994-11-01), Giese
patent: 5516636 (1996-05-01), McCapra
patent: 5516931 (1996-05-01), Giese
patent: 5536834 (1996-07-01), Singh
patent: 5578498 (1996-11-01), Singh
patent: 5602273 (1997-02-01), Giese
patent: 5604104 (1997-02-01), Giese
patent: 5610020 (1997-03-01), Giese
patent: 5616719 (1997-04-01), Davalian
patent: 5622929 (1997-04-01), Willner
patent: 5635602 (1997-06-01), Cantor
patent: 5650270 (1997-07-01), Giese
patent: 5677171 (1997-10-01), Hudziak et al.
patent: 5705622 (1998-01-01), McCapra
patent: 5709994 (1998-01-01), Pease
patent: 5766481 (1998-06-01), Zambias
patent: 5777096 (1998-07-01), Grossman
patent: 5789172 (1998-08-01), Still
patent: 5807675 (1998-09-01), Davalian
patent: 5843655 (1998-12-01), McGall
patent: 5843666 (1998-12-01), Akhavan-Tafti
patent: 5846839 (1998-12-01), Gallop
patent: 5849878 (1998-12-01), Cantor
patent: 5898005 (1999-04-01), Singh
patent: 5952654 (1999-09-01), Giese
patent: 5958202 (1999-09-01), Regnier
patent: 6027890 (2000-02-01), Ness
patent: 6251581 (2001-06-01), Ullman
patent: 6322980 (2001-11-01), Singh
patent: 6331530 (2001-12-01), Breslow
patent: 6346384 (2002-02-01), Pollner
patent: 7105308 (2006-09-01), Chan-Hui et al.
patent: 7135300 (2006-11-01), Chan-Hui et al.
patent: WO 00/66607 (2000-11-01), None
patent: WO 01/90399 (2001-11-01), None
Giese, “Electrophoric Release Tags: Ultrasensitive Molecular Labels Providing Multiplicity”, Trends in Analytical Chemistry, vol. 2, No. 7, 1983, pp. 166-168.
Kochevar et al., “Photosensitized Production of Singlet Oxygen”, Methods in Enzymology, vol. 319, 2000, pp. 20-29.
Ni et al., “Versatile Approach to Encoding Combinatorial Organic Synthesis Using Chemically Robust Secondary Amine Tags”, J. Med. Chem., vol. 39, 1996, pp. 1601-1608.
Olejnik et al., “Photocleavable Affinity Tags for Isolation and Detection of Biomolecules”, Methods in Enzymology, vol. 291, 1998, pp. 135-154.
Oseroff et al., “Antibody-Targeted Photolysis: Selective photodestruction of Human T-Cell Leukemia Cells Using Monoclonal Antibody-Chlorine6Conjugates”, Proc. Natl. Acad. Sci. USA, vol. 83, 1986, pp. 8744-8748.
Rakestraw et al., “Antibody-Targeted photolysis: In vitro Studies with Sn(IV) Chlorin e6 Covalently Bound to Monoclonal Antibodies Using a Modified Dextran Carrier”, Proc. Natl. Acad. Sci. USA, vol. 87, 1990, pp. 4217-4221.
Ullman et al., “Luminescent Oxygen Channeling Immunoassay: Measurement of Particle Binding Kinetics by Chemiluminescence”, Proc. Natl. Acad. Sci. USA, vol. 91, 1994, pp. 5426-5430.
Beaudet et al, “Homogeneous Assays for Single-Nucleotide Polymorphism Typing Using AplhaScreen”, Genome Research, 11-600-608, 2001.
Angers et al, “Dimerization: An Emerging Concept for G Protein-Coupled Receptor Ontogeny and Function”, Annu. Rev. Parmacol. Toxicol, (2002) 42-409-435.
Overton et al, “G-protein-coupled Receptors Function as Oligomers In Vivo”, Current Biology, (2000) vol. 10, No. 6, 341-344.
Mellado et al, “Chemokine Signaling and Functional Responses: The Role of Receptor Dimerization and TK Pathway Activation” Annu. Rev. Immunol., 2001, 19,397-421.
Gomes et al, “G Protein Coupled Receptor Dimeraztion: Implications in Modulating Receptor Function”, J. Mol. Med., 2001, 79,, 226-242.
Salim et al, “Oligomerization of G-protein-coupled Receptors Shown by Selective Co-immunoprecipitation”, Journal of Bioligical Chemistry, 2002, vol. 277, No. 18, Issue of May 3, 2002, 15482-15485.
Angers et al, “Detection of β2-Adrenergic Receptor Dimerization in Living Cells Using Bioluminescence Resonance Energy Transfer (BRET)”, PNAS, Mar. 28, 2000, vol. 97, No. 7, 3684-3689.
Jones et al., “Signal Transduction by GABABReceptor Heterodimers”, NeuroPsychopharmacology, 2000, ?vol. 23, No. S4, S41-S49.
Jordan et al., “G-protein-coupled Receptor heterodimerization Modulates Receptor Function”Nature, Jun. 17, 1999, vol. 399, 697-700.
McVey et al, “Monitoring Receptor Oligomeirzation Using Time-resolved Fluorescence Resonance Energy Transfer and Bioluminescence Energy Transfer”, The Journal of Biological Chemistry, Apr. 27, 2001, vol. 276, No. 17, 14092-14099.
Devi, “Heterodimerization of G-protein -coupled Receptors: Pharmacology, Signaling and Trafficking”, Trends in Pharmacological Science, Oct. 2001, vol. 22, No. 10, 532-537.
George et al, “G-protein-coupled Receptor Oligomerization and Its Potential for Durg Discovery”, Nature Reviews/Drug Discoveries, Oct. 2002, vol. 1, 808-820.
Rios et al, “G-protein-coupled Receptor Dimerization: Modulatio of Receptor Function”, Pharmacology & Therapeutics, 2001, vol. 92, 71-87.
Schlessinger, Ligan'induced, Receptor-mediated Dimerization and Activation, Cell, Sep. 20, 2002, vol. 110, 669-672.
Chan-Hui Po-Ying
Dua Rajiv
Pidaparthi Sailaja
Shi Yining
Singh Sharat
Fenwick & West LLP
Haq Shafiqul
Le Long V.
Monogram BioSciences, Inc.
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