Measuring apparatus using enzyme electrodes and the method there

Details Measuring apparatus using enzyme electrodes and the method there Measuring apparatus using enzyme electrodes and the method there

1991-07-08

1993-07-06

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435280, 435291, 204403, 20415312, 422 8203, G01N 27327, G01N 33573, G01N 3366

Patent

active

052253210

ABSTRACT:
In the case of measuring concentration of, for example, glucose as a first substance to be measured, and that of sucrose as a second substance to be measured, using a measuring apparatus, the apparatus is provided with a first enzyme electrode, a dilution pipeline situated at the downstream of the electrode and for diluting the substances, and a second enzyme electrode situated at the downstream of the dilution pipeline. A sample, which contains the first and second substances, is poured intermittently into a constant flow of a buffer solution. The first enzyme electrode detects the first substance. The second enzyme electrode, which comprises an immobilized enzyme catalizing the conversion of the second substance in to the first substance, detects the first substance, which is originally contained in the sample as well as is formed on the second enzyme electrode. The concentration of the second substance in the sample can be determined by calculating about the outputs of the first and second enzyme electrodes.

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