Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1994-08-04
1997-07-15
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
435212, 435214, 436 69, C12Q 156, G01N 3386
Patent
active
056482280
ABSTRACT:
The present invention provides a method for measuring the activity of tested substances utilizing a reconstituted plasma kallikrein-kinin system. A series of enzymatic reactions is started wherein an activation of a blood coagulation factor XII is an initiating reaction. The series of reactions is started in the presence of the tested substance in the reconstituted plasma kallikrein-kinin system. Then, the series of reactions is stopped and the physiologically active substance produced in the reaction series is quantitatively determined. In the activity measuring method of the present invention, the real activity (a promoting or inhibiting ability) of the tested substance against the production of the produced physiologically active substance can be easily, simply, quickly and precisely measured. In the reconstituted plasma kallikrein-kinin reaction system the contaminating other factors are substantially removed. Accordingly, the method of measuring the activity in accordance with the present invention is a very advantageous means for ascertaining action which is useful for adjusting the bioregulations in, for example, the plasma kallikrein-kinin system, the blood clotting system, the fibrinolysis system, etc. It may also be used for developing or screening drugs which are related to the systems.
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Kaplan, A., "Assessment of Hageman Factor Activation in Human Plasma: Quantification of Activated Hageman Factor-C1 Inactivator Complexes by an Enzyme-Linked Differential Antibody Immunosorbent Assay" Blood, vol. 66, No. 3 (Sep.) 1985, pp. 636-641.
Gillmore, M.J., A Direct Chromogenic Peptide Substrate Assay for Hageman Factor (FXII), J. of Fibrinolysis, 1(2), 1987, pp. 123-127.
Dumenco, L, Inhibition of the activation of Hageman factor (factor XII) by platelet factor 4, J. Lab. Clin. Med., 112(3), 1988, pp. 394-400.
Kawakubo Hitoshi
Nishikawa Katsumi
Gitomer Ralph
Nippon Zoki Pharmaceutical Co. Ltd.
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