Optics: measuring and testing – By particle light scattering – With photocell detection
Patent
1983-05-24
1986-07-22
Kittle, John
Optics: measuring and testing
By particle light scattering
With photocell detection
G01N 2149
Patent
active
046015787
ABSTRACT:
A micro-sample of semen is exposed to a beam of light from an inexpensive and small laser device. Light scattered by the sample is detected by a photodetector. The photodetector signal is analyzed in the frequency domain to provide a measure of the amplitude of the signal components having frequencies above about 100 Hz. This measure is representative of the numbers of motile sperm in the sample. A motile/immotile percentage is obtained by dividing the motile measurement by a value representative of the amplitude of the full-frequency photodetector signal. In a preferred embodiment frequency-amplitude analysis is carried out in the time domain using frequency splitting filters and integrating the filtered signals. The light receiving aperture for the photodetector is made annular or part-annular in shape to overcome sample orientation or polarization effects.
REFERENCES:
Naylor et al., Apparatus for the Study of Motile Sperm Using Microprocessor Analysis of Scattered Laser Light, Med, & Biol. Eng. & Comput., 20, 207-214 (Mar. 1982).
Jouannet et al., Light Scattering Determination of Various Characteristic Parameters of Spermatozoa Motility in a Serie of Human Sperm, Andrologia 9(1), 36-49 (1977).
Woolford Murray W.
Woolhouse James K.
Kittle John
Saitta Thomas C.
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