Measurement of fluorescence using capillary isoelectric...

Details Measurement of fluorescence using capillary isoelectric... Measurement of fluorescence using capillary isoelectric...

2005-02-08

2005-02-08

Choi, Ling-Siu (Department: 1713)

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

C204S601000, C204S603000, C204S612000

Reexamination Certificate

active

06852206

ABSTRACT:
Improved apparatus is disclosed for carrying out axially illuminated laser induced fluorescence whole-column imaging detection in the capillary isoelectric focusing of proteins. The separation capillary was made of low refractive index Teflon conditioned with methylcellulose to reduce electroosmotic flow and a small amount of high refractive index organic solvent (glycerol) was added to the sample mixture. It was found that an axially directed laser excitation beam was propagated essentially with total internal reflection, so that minimum interference arose from stray light or from scattering light originating from the wall of the capillary. With the naturally fluorescent protein R-phycoerythrin, a concentration detection limit LOD 10−11M or mass LOD 10−17Mo was obtained.

REFERENCES:
patent: 5324401 (1994-06-01), Yeung et al.
Huang et al., “Axially illuminated fluorescene imaging detection for capillary isoelectric focusing on Teflon capillary”, The Analy Communication, 125, 1231-1233(2000).*
Johansson, “Fluorescene imaging of light absorption for axially-beam geomtry in capillary electrophorescene”, Electrophoresis 19, 2233-2238(1998).*
Taylor et al., “Axial-beam laser-excited fluorescence detection in capillary electrophoresis”, Anal. Chem., 64, 1741-1744(1992).

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