Measurement of feed digestibility in ruminants

Chemistry: analytical and immunological testing – Food or dairy products

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436 171, 426 2, 426231, 422 8205, G01N 3100, G01N 3302, G01N 2129, A01K 2900, A01K 4300

Patent

active

060080531

DESCRIPTION:

BRIEF SUMMARY
The present invention relates to a novel process for determining the nutritional value of silage given to ruminants. It has long been sought to optimize the nutritional value of feeds given to reared animals in order to increase, in particular in ruminants, milk and/or meat production.


DESCRIPTION OF THE RELATED ART

It is known, for example according to the publications published in Can. J. Anim. Sci. 67;557-562 (Jun. 1987) that it is possible to determine both the "in vitro" digestibility of various corn hybrids and the same digestibility by a method of determination by near-infra-red spectrophotometry.
It is also known, according to the article from the following authors: Valdes, Hunter and Pinter, published in Can. J. Plant Sci. 67;747-754 (Jul. 1987) to determine the IVMD (in vitro dry-matter digestibility) values which were hitherto obtained by an "in vitro" ruminal fermentation technique described by Tilley and Terry in 1963, by a method of near-infra-red spectrophotometric analysis.
The methods described in the above two publications have made it possible to combine "in vitro" digestibility analysis methods with near-infra-red spectrophotometric analysis methods. This combination for estimating the "in vivo" digestibility of chemical and enzymatic analysis methods, which themselves have large variabilities, with a near-infra-red spectrophotometric method which itself also has a large variability made it possible to obtain fairly moderate evaluations of the digestibility.
As regards the prediction of the "in vivo" digestibility by near-infra-red spectrophotometric analysis, no publication has appeared to date. Other publications combining "in vitro" analyses with near-infra-red spectrophotometry have been published, such as the publication by Dardenne, Andrieu, Barriere, Biston, Demarquilly, Femenias, Lila, Maupetit, Riviere and Ronsin, Ann. Zootech (1993) 42, 251-270 which established calibration curves for the following analyses: dry matter, ash, total proteins, water-soluble carbohydrates, celluloses, cell wall constituents, total fibre, acidic and neutral detergent fibre, lignin, enzymatic solubilities, determined by the methods of Aufrere, Limagrain and Lila. These various "in vitro" analyses make it possible to determine, as above, an "in vivo" digestibility value. These "in vitro" analyses have all been made possible by means of a determination by near-infra-red spectrophotometry, which has made it possible by calculation to determine an "in vivo" digestibility value.
None of these methods has hitherto combined an "in vivo" determination of the nutrient value of a silage directly with an analysis by near-infra-red spectrophotometry. This determination was not obvious on reading any of this essentially chemical and mathematical prior art, since many physiological phenomena occur in an animal's body which have never been taken into account in the chemical or enzymatic analyses performed to date and which have thus not been able to lead to a determination by near-infra-red spectrophotometry.


BRIEF SUMMARY OF THE INVENTION

The object of the present invention is the determination of the "in vivo" nutrient value of feed in ruminants by near-infra-red spectrophotometry. The invention relates more particularly to the determination of the ruminal degradability value of fodder in ruminants. The invention may also relate to the intestinal digestibility value of amino acids. Thus, this method can make it possible to determine the actual degradability value of feed during its transit through the digestive system.


BRIEF DESCRIPTION OF THE DRAWING

FIG. 1 is a calibration curve for DMS titled "In-sachet" degradability (%) vs. N.I.R. predictions (%).


DETAILED DESCRIPTION OF THE INVENTION

Among the feeds whose nutritional value and ruminal degradability value can be determined by near-infra-red analysis, mention may be made of silage and most particularly of grass silage, corn silage, alfalfa silage, spent grain silage and beetroot pulp silage. These silages are preferably stored in minisilos contai

REFERENCES:
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patent: 5728920 (1998-03-01), Leudtke et al.
patent: 5747020 (1998-05-01), Rutherford et al.
Sanderson et al. "Use of Near Infrared Reflectance Spectroscopy to Predict and Compare the Composition of Corcaso Sapler From Young Steers" Animal Science 65 (1), 45-54, 1997.
Sinnaeve et al "The Use of Near Infrared-Spectroscopy for the Analysis of Fresh Grass-Silage". J. Near Infrared Spectrose. 2(2) 79-84 Abstract only, 1994.
Hsu et al. Use of Near Infrared Spectroscopy for Determining Protein Fractions in Alfalfa. Near Infrared Spectrosc. Future Wavas, Proc. Int. Conf. Near Infrared Spechosc. 7.sup.th (1996) 581-587, 1996.
Park et al. The Use of Near Infrared Reflectace Spectroscopy on Dried Saple to Predict Biological Parameters of Grass Silage. Animal Feed Sci. Techol. 68(3-4) 235-246, 1997.
Amari et al Estimation of Chemical Composition and Nutritive Value of Forages Nnear Infrared Reflectance Spectroscopy. Nippon Sochi Gakkeishi 34 (4) 271-9, 1989.
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De La Roza et al The Use of Near Infrared Reflectance Spectroscopy to Predict the Nutritive Value and in Vivo Digestibility of Grass Silages Making Light Work: Adv. Near Infrared Spectrosc. Int. Conf. Near Infrared Spectrosc, Meeting Date 1991, 269-71. Editors Murray et al; Publisher VCH, Weinheim Germany, 1992.
Antoniewicz et al., "Rumen Degradability of Crude Protein of Dried Grass and Lucerne Forage Measured by in Sacco Incubation and Predicted by Near Infrared Spectroscopy,"Animal Feed Science and Technology, 54:203-216 (1995).
Dardenne et al., "Composition and Nutritive Value of Whole Maize Plants Fed Fresh to Sheep. II. Prediction of the in vivo Organic Matter Digestibility," Annals of Zootech 42:(3):251-270 (1993).
Downey et al., "Dried Grass Silage Analysis by Nir Reflectance Spectroscopy--a Comparison of Stepwise Multiple Linear and Principal Component Techniques for Calibration Development on Raw and Transformed Spectral Data," Journal of Chemometrics, 3(2):397-407 (1989).

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