Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1995-12-27
1996-10-15
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 18, 435 29, 435 34, 435 37, 435968, 436172, C12Q 126, H01L 21306
Patent
active
055653286
ABSTRACT:
The present invention relates to a method to determine the concentration of an unknown substance in a colorimetric reaction using a fluorometric reader. The change in color can be monitored by observing the change of fluorescence of a known amount of a suitable fluorophore in the reaction chamber. Under appropriate conditions the absorption spectrum of the chromophore overlaps with the emission spectrum of the fluorophore thereby allowing the change in fluorescence to be proportional to the intensity of color in the reaction and thus, proportional to the quantity of the substance of interest. Specifically, 8-methoxypyrene tri-sulfonic acid is used as the fluorophore.
REFERENCES:
patent: 4174384 (1979-11-01), Ullman et al.
patent: 4495293 (1985-01-01), Shaffar
patent: 4743561 (1988-05-01), Shaffar
patent: 4912035 (1990-03-01), Belly et al.
patent: 4954435 (1990-09-01), Krauth
Nolte et al., J. Clin. Mirobio., 26:1079-1084 (1988).
Godsey et al., J. Clin. Microbio., 13:483-490 (1981).
8th Edition-Fundamentals of Microbiology Martin Frobisher, ScD., W. B. Saunders Company, pp. 266-268 (1968).
Bascomb, S. Meth in Microbiol. 19:105-160, 1987.
Sykes, R. B., and Nordstrom, K. Antimicrobial Agents and Chemotherapy 1(2):94-99, 1972.
Carmel, A., Yaron, A. "An Intramolecularly Quenched Fluorescent Tripeptide as a Fluorogenic Substrate of Angiotensin-I-Converting Enzyme and of Dipeptidyl Carboxypeptidase", Eur. J. Biochem, 87, 265-273 (1978).
Carmel, A., Kellser, E., Yaron, A., "Intramolecularly-Quenched Fluorescent Peptides as Fluorogenic Substrates of Leucine Aminopeptidase and Inhibitors of Clostridial Aminopeptidase", J. Biochem, 73, 617-625 (1977).
Fleminger, G., Carmel, A., Goldenberg, D., Yaron, A., "Fluorogenic Substrates For Bacterial Aminopeptidase P and Its Analogs Detected in Human Serum and Calf L Lung," Eur. J. Biochem, 125, 609-615 (1982).
Ando, T. et al., "Pyruvate as a Fluorescence Quencher: A New Spectroscopic Assay for Pyruvate Reactions", Anal. Biochem. 129, 170-175 (1983).
Bascomb, S., "Enzyme Tests in Bacterial Identification", Methods in Microbiol., vol. 19, ISBN 0-12-521519-3, 105-160 (1987).
Blumberg, W. E., Doleiden, F. H. and Lamola, A. A., Hemoglobin Determined in 15 ul of Whole Blood by "Front Face" Fluorometry, Clin. Chem. 26/3, 409-413 (1980).
Florentin, D., Sassi, A. Roques, B. P., "A Highly Sensitive Fluorometric Assay for Enkephalinase, a Neutral Metalloendopeptidase That Releases Tyrosine-Glycine-Glycine from Enkephalins", Anal. Biochem. 141, 62-69 (1984).
Toro, G., Ackermann, P. G., "Practical Clinical Chemistry", c. 1975 Little Yaron, A., Carmel, A., Katchalski-Katzir, E., "Intramolecularly Quenched Fluorogenic Substrates for Hydrolytic Enzymes,", Anal. Biochem. 95, 228-235 (1979).
Bascomb Shoshana
Sand Ted
Swenson Frank J.
Dade International Inc.
Mohamed Abdel A.
Tymeson Cynthia G.
Weimar Elizabeth C.
LandOfFree
Measurement of color reactions by monitoring a change of fluores does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Measurement of color reactions by monitoring a change of fluores, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Measurement of color reactions by monitoring a change of fluores will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1245062