Measurement of bacterial CO.sub.2 production in an isolated fluo

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

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422 52, 422 55, 422 56, 422 8205, 422 8207, 435 34, 435 39, 435291, 435808, 436167, 436169, 436170, 436172, G01N 2176

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active

053727843

ABSTRACT:
A multi-layer blood culture sensor includes two matrices. The first matrix is a polymer that is permeable to carbon dioxide and water, but impermeable to protons. A pH sensitive absorbance based dye is encapsulated or isolated in the polymer. The second matrix is a polymer with a pH insensitive fluorescent dye encapsulated or isolated in the polymer. The matrices are spectrally coupled and are useful for the determination of microorganisms in a blood culture bottle.

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