Measurement of angular dependence of scattered light in a flowin

Details Measurement of angular dependence of scattered light in a flowin Measurement of angular dependence of scattered light in a flowin

1975-06-05

1976-06-01

Corbin, John K.

Optics: measuring and testing

Refraction testing

Prism engaging specimen

250574, G01N 2100

Patent

active

039604497

ABSTRACT:
Particle analyzing method and apparatus are provided for measuring the intensity of light scattered from a particle as a function of angle. Particles such as biological cells are contained in a stream for travel along a path which includes a scatter line along which the particles pass successively. A beam of collimated monochromatic radiation is directed onto the flow path, and detector means are provided having a radiation acceptance geometry whose projection intercepts the beam along the scatter line. The projected acceptance geometry includes an aperture image located adjacent the scatter line and having a dimension in the direction of travel of the particles which is substantially less than the length of the scatter line such that the angle of scatter radiation detectable by the detector means varies according to the position of the particle along the scatter line. The output from the detector provides a measure of the scatter light intensity versus angle characteristic of the particle.

REFERENCES:
patent: 3422667 (1969-01-01), Hrdina
patent: 3713743 (1973-01-01), Simms
patent: 3822095 (1974-07-01), Hirschfeld
patent: 3835315 (1974-09-01), Gravitt, Jr.
patent: 3869209 (1975-03-01), Sigrist

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