Measurement of analyte concentration

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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436172, 436518, 436528, 436532, 436546, 436810, 435 71, 435 78, 4353091, 435805, G01N 33566, G01N 2176, G01N 33543, G01N 3353

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active

055997201

ABSTRACT:
A method of measuring the ambient concentration in a fluid, especially a body fluid, of an analyte, such as a hormone or other biologically active material, whose concentration in the fluid is dependent on thedilution of the fluid is disclosed. The fluid is contacted with a trace amount of a binding agent, such as an antibody, specific for the analyte, the proportional occupancy of binding sites on the binding agent is determined and from that figure the analyte concentration is estimated without the need to measure accurately beforehand the volume of the fluid or fluid sample being tested. It is therefore possible to design a concentration-measuring device for insertion into a body fluid of a living creature for in situ measurement of concentration.

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