Measurement of alkaline phosphatase levels in body fluids

Details Measurement of alkaline phosphatase levels in body fluids Measurement of alkaline phosphatase levels in body fluids

1976-09-10

1977-11-22

Tanenholtz, Alvin E.

Textiles: fiber preparation

1951035R, 195127, 204 1T, G01N 3114, G01N 3300

Patent

active

040594904

ABSTRACT:
A method of measuring the alkaline phosphatase level in a body fluid such as serum or plasma comprising hydrolyzing excess monophosphate ester dissolved in a suitable buffer by action of alkaline phosphatase in a predetermined volume of the body fluid to yield an oxidizable substrate, oxidizing the substrate with excess molecular oxygen in the presence of excess oxygenase and measuring the rate of oxygen consumption, preferably by means of an oxygen electrode. The invention also provides a reagent for use in this method which comprises a diethanolamine-HCl buffer having a molarity of 0.1 M to 4.0 M and a pH of 8.6 to 10.8 and containing magnesium ions in a concentration of 100 to 1000 .mu.M and an excess of oxygenase.

REFERENCES:
patent: 3791929 (1974-02-01), Hammer
patent: 3933593 (1976-01-01), Sternberg
Bergmeyer, "Methods of Enzymatic Analysis", 2nd Ed., vol. 2, Academic Press, Inc., N.Y. (1974), pp. 864-866.

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