Measurement method of peroxidase activity

Details Measurement method of peroxidase activity Measurement method of peroxidase activity

1986-12-01

1989-08-08

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435188, 435810, C12Q 128, C12N 996

Patent

active

048552291

ABSTRACT:
A measurement method of peroxidase activity is based on reaction of o-phenylenediamine and hydrogen peroxide in the presence of peroxidase. This method includes preparing and storing a first reagent containing o-phenylenediamine and having a first pH value higher than a second pH value at which the reaction is conducted, and preparing and storing a second reagent having a buffering capability larger than the first reagent. At the time of measurement, the stored first and second reagents are mixed together with a sample containing peroxidase, and a predetermined amount of hydrogen peroxide to provide a reaction mixture having the second pH value. The reaction is conducted in the reaction mixture, thereby producing 2,2'-diamino-azobenzene, and the absorbance increase at the maximum absorption wavelength of the produced 2,2'-diamino-azobenzene is measured.

REFERENCES:
patent: 4193938 (1980-03-01), White
patent: 4728608 (1988-03-01), Roberts et al.
Porstmann et al., J. Clin. Chem. Clin. Biochem., vol. 23 (1985), pp. 41-44.

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