Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1996-11-15
2000-11-21
Spector, Lorraine
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530395, 530397, 436 63, A61K 3816
Patent
active
061503266
DESCRIPTION:
BRIEF SUMMARY
FIELD OF TECHNOLOGY
The present invention relates to medicine, more specifically to the use of immune correcting preparations for the treatment of autoimmune diseases.
The present invention can be used as a medication for diagnosing a suppressor component of human immune status and for evaluating the possibility of treating autoimiune diseases, in particular, disseminated sclerosis, rheumatic arthritis, etc.
PRIOR ART
Known is .beta.-I-qlycoprotein of placental origin which is a of trophoblastic .beta.-I-glycoprotein (TBG), used as a growth and proliferation stimulator for hematopoietic blood cells (U.S. Pat. No. 5,169,835, cl. A61K 35/50, 1989).
Nevertheless the known compound has not been used as a means for treating of autoimmune diseases.
Known is the use of TBG for diagnosing and prognosticating the course of pregnancy (see L.G. Sotnikova, I.N. Golovistikov et al., The Role of .beta.-I-glycoprotein Trophoblast in Diagnosing and Prognosticating Pregnancy, Metodicheskije Recomendatsii, Moscow, 1984).
Nevertheless said work does not disclose the possibility of using TBG for diagnosing a suppressor component and treating autoimmune diseases.
Known is the method for treating pustular psoriasis by administering a drug of placental origin (see the USSR Inventor's Certificate No.106181, cl. A61K 35/50 as of Jul. 19, 1982)
Nevertheless the use of the drug according to said method fails to prevent side effects which may occur due to impurities.
Known is the method for treating autoimmune diseases comprising administering of a dosage of an immune correcting preparation of placental origin (see the USSR Inventor's Certificate No. 1657190, cl. A61K 35/50 as of 1989).
Nevertheless said method employs a preparation containing impurities which may cause allergic reactions, moreover said preparation is effective only during postsurgical period and is limited to autoimmune orchitis.
SUMMARY OF THE INVENTION
The main object of the present invention is to provide an effective method of treatment of autoimmune diseases by administering a preparation which shows an immune correcting activity and does not cause side effects, as well as to broaden the range of autoimmune diseases which can be treated therewith.
Said object is achieved by new use of trophoblastic .beta.-I-glycoprotein (TBG) as a means for treating autoimmune diseases, and the method of treatment of autoimmune diseases comprising the administration of the immune correcting preparation according to the invention, additionally comprises a prestudy of immune status, and in case a deficit of suppressors is determined, then trophoblastic .beta.-I-glycoprotein (TBG) is used as an immune correcting preparation.
The prestudy of immune status may comprise collecting a sample of peripheral blood, obtaining mononuclear cells (MNCs), dividing the sample into two portions, cultivating the first portion without TBG, and cultivating the second one with TBG, washing MNCs out of culture medium, blocking proliferation, adding into each of said MNC portions newly isolated MNCs obtained from a normal donor, which latter MNCs having been stimulated with phytohemagglutinin in equal proportions to produce test cultures, cultivating said cultures, further evaluating the proliferation of said cultures and determining the suppression values based on the ratios of the levels of proliferation in test cultures.
Experiments and clinical tests have shown new properties of TBG as an immune correcting preparation useful for treating different autoimmune diseases.
The study of immune status values for 154 patients suffering autoimmune diseases (disseminated sclerosis, rheumatic arthritis, etc.) has shown T-suppressors deficit, which is starting mechanism for autoimmunization, i.e. for the loss of autotolerance.
The treating effect of TBG based on its ability to induce suppressive activity of lymphocytes in patients suffering disseminated sclerosis, rheumatic arthritis, etc.
Clinical immunological surveys carried out on the patients suffering abovementioned diseases and on a control grou
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Gorlina et al, The Soviet Journal of Developmental Biology (English translation of Ontogenez) 14 (6):366-369, Sept. 1984.
Effect on Pregnancy and Hormonal Changes on the Activity of Rheumatoid Arthritis Monika Ostensen, et al, Dept. of Rheumatology, Institute of Clinical Medicine, Univ. of Tromso, Norway, Scand J. Rheum. 12:69-72, 1983.
Suppressor Cell Activity After Concanavalin a Treatment of Lymphocytes from Normal Donors, Shou, et al, Journal of Experimental Medicine vol. 143, 1100-1110; 1976.
Inhibitory and Stimulatory Effects of Concanavalin on the Response of Mouse Spleen Cell Suspensions to Antigen, Richard Dutton, Journal of Experimental. Medicine vol. 138, pp. 1496-1505 (1973).
Immunochemical Identification of New Beta-1-Globulin in the Blood Serum of Pregnant Women, Bulletin of Experimental Biology and Medicine, 1970 No. 6, Medicina Publishers, Moscow, pp. 66-68, Figs. 1-2.
Human Trophoblastic Beta-1-Globulin and Chorionepithelioma, Tatarinov, et al, Onco Developmental Gene Expression, 1976, pp. 463-468.
Alikhanov Khallar Abdumuslimovich
Golovistikov Ivan Nikolaevich
Kacharava Leonid Yazonovich
Tatarinov Jury Semyonovich
Golovistikov Ivan Nikolaevich
Kacharava Leonid Yazonovich
Lazar-Wesley Eliane
Spector Lorraine
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