Means for separating lymphocytes and monocytes from anticoagulat

Details Means for separating lymphocytes and monocytes from anticoagulat Means for separating lymphocytes and monocytes from anticoagulat

1978-07-10

1980-02-26

Hart, Charles N.

Liquid purification or separation

With alarm, indicator, register, recorder, signal or...

Responsive to fluid flow

210DIG23, B01D 2126

Patent

active

041905350

ABSTRACT:
This invention relates to a method for isolating platelets, lymphocytes, and monocytes from anticoagulated blood. The method involves centrifuging at high force a sample of uncoagulated blood with a water-insoluble thixotropic gel-like material having a specific gravity between 1.065-1.077 g/cc for a length of time sufficient to cause the gel-like material to form a barrier between the platelets, lymphocytes, monocytes, and plasma fraction of the blood and the heavier blood cells. Thereafter, the plasma is withdrawn and the platelets, lymphocytes, and monocytes removed from above the barrier in a buffer solution. Where desired, the platelets can be separated from the lymphocytes and monocytes utilizing a similar process but wherein the water-insoluble thixotropic gel-like material has a specific gravity of less than about 1.055 g/cc.

REFERENCES:
patent: 3780935 (1973-12-01), Lukacs et al.
patent: 3852194 (1974-12-01), Zine
patent: 3920549 (1975-11-01), Gigliello et al.
patent: 3957654 (1976-05-01), Ayres
patent: 3963119 (1976-06-01), Lukacs et al.
patent: 4021340 (1977-05-01), Zine
patent: 4027660 (1977-06-01), Wardlaw et al.
patent: 4049692 (1977-09-01), Zine
patent: 4082085 (1978-04-01), Wardlaw et al.
patent: 4083784 (1978-04-01), Zine
patent: 4083788 (1978-04-01), Ferrara
Zucker et al., "The Separation of Normal Human Leukocytes by Density and Classification by Size", Blood, vol. 34, No. 5, pp. 591-600, (1969).

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