Means for enhancing gene expression

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4353201, 435419, C07H 2104, C12N 1500

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active

059553308

ABSTRACT:
The present invention provides a novel means for enhancing the expression of genes by the use of the first intron of the Zea mays Sh1 cDNA, functional sequences derived therefrom and/or sequences flanking the first Sh1 intron, placed between the transcription start site and the translation start site of the coding sequence for enhanced expression is sought. The DNA sequences provided herein can be used, for example, to increase protein expression in engineered plant cells or in transgenic plants.

REFERENCES:
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Sheldon, E., R. Ferl, N. Fedoroff, and L.C. Hannah (1983) "Isolation and Analysis of a Genomic Clone Encoding Sucrose Synthetase in Maize: Evidence for Two Introns in Sh," Mol. Gen. Genet. 190:421-426.
Zack, C.D., R.J. Ferl, L.C. Hannah (1986) "DNA sequence of a shrunken allele of maize: Evidence for visitation by insertional sequences," Maydica XXXI:5-16.
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Hauptmann, R.M., P. Ozias-Akins, V. Vasil, Z. Tabaeizadeh, S.G. Rogers, R.B. Horsch, I.K. Vasil, and R.T. Fraley (1987) "Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species," Plant Cell Reports 6:265-270.
Hauptmann, R.M., M. Ashraf, V. Vasil, L.C. Hannah, I.K. Vasil, and R. Ferl (1988) "Promoter Strength Comparisons of Maize Shrunken 1 and Alcohol Dehydrogenase 1 and 2 Promoters in Mono-and Dicotyledonous Species," Plant Physiol. 88:1063-1066.
Chee, P.P., R.C. Klassy, and J.L. Slighton (1986) "Expression of a bean storage protein `phaseolin minigene` in foreign plant tissues," Gene 41:47-57.
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