Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1991-08-14
1999-03-09
Warden, Robert J.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204458, 204603, 204606, G01N 2726, G01N 27447
Patent
active
058795286
ABSTRACT:
A means and method for indirect detection of constituent components of a mixture separated in a chemical separation process. Fluorescing ions are distributed across the area in which separation of the mixture will occur to provide a generally uniform background fluorescence intensity. For example, the mixture is comprised of one or more charged analytes which displace fluorescing ions where its constituent components separate to. Fluorescing ions of the same charge as the charged analyte components cause a displacement. The displacement results in the location of the separated components having a reduced fluorescence intensity to the remainder of the background. Detection of the lower fluorescence intensity areas can be visually, by photographic means and methods, or by automated laser scanning.
REFERENCES:
Yinfa Ma & Edward S. Yeung "Indirect Flurometric Detection of Anions in Thin-layer Chromatography" Analytical Chemistry, 60, (1988), 722-724.
Carmen W. Huie & William R. Williams "Laser Fluorometric Detection of Porphyrin Methyl Esters for High-Performance Thin-Layer Chromatography" Analytical Chemistry, 61, (1989), 2288-2292.
Yinfa Ma, Lance B. Koutny, & Edward S. Yeung "Laser-Based Indirect Fluorometric Detection and Quantitation in Thin-Layer Chromatography" Analytical Chemistry, 61, (1989), 1931-1933.
B. G. Belenkii et al "Use of a Scanning Laser Fluorometric Detector in High-Performance Thin Layer Chromatography" Journal of Chromatography, 365, (1986), 315-320.
Chan King C.
Hogan Barry L.
Koutny Lance B.
Ma Yinfa
Yeung Edward S.
Iowa State University Research Foundation
Starsiak Jr. John S.
Warden Robert J.
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