Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1990-01-30
1993-03-09
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 27447, B01D 5702
Patent
active
051924072
ABSTRACT:
A means and method for indirect detection of constituent components of a mixture separated in a chemical separation process. Fluorescing ions are distributed across the area in which separation of the mixture will occur to provide a generally uniform background fluorescence intensity. For example, the mixture is comprised of one or more charged analytes which displace fluorescing ions where its constituent components separate to. Fluorescing ions of the same charge as the charged analyte components cause a displacement. The displacement results in the location of the separated components having a reduced fluorescence intensity to the remainder of the background. Detection of the lower fluorescence intensity areas can be visually, by photographic means and methods, or by automated laser scanning.
REFERENCES:
Yinfa Ma & Edward S. Yeung, "Indirect Fluorometric Detection of Anions in Thin-Layer Chromatography" Analytical Chemistry, vol. 60, No. 7 (Apr. 1, 1988) 722-724.
Yinfa Ma & Edward S. Yeung, "Indirect Fluorimetric Detection of Non-Electrolytes in Thin-Layer Chromatography" Journal of Chromatography, vol. 455 (1988) 382-389.
Barry L. Hogan and Edward S. Yeung, "Indirect Fluorometric Detection in Gel Electrophoresis" Applied Spectroscopy, vol. 43 No. 2 (1989) 349-350.
Yinfa Ma, Lance B. Koutny, & Edward S. Yeung, "Laser-Based Indirect Fluorometric Detection and Quantification in Thin-Layer Chromatography" Analytical Chemistry, vol. 81, No. 17 (1989) 1931-1933.
Cheung Chan K.
Hogan Barry L.
Koutny Lance B.
Ma Yinfa
Yeung Edward S.
Iowa State University & Research Foundation, Inc.
Niebling John
Starsiak Jr. John S.
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