Means and method for capillary zone electrophoresis with laser-i

Details Means and method for capillary zone electrophoresis with laser-i Means and method for capillary zone electrophoresis with laser-i

1989-02-06

1991-04-09

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, B01D 5702

Patent

active

050062107

ABSTRACT:
A means and method for capillary zone electrphoresis with laser-induced indirect fluorescence detection. A detector is positioned on the capillary tube of a capillary zone electrophoresis system. The detector includes a laser which generates a laser beam which is imposed upon a small portion of the capillary tube. Fluorescence of the elutant electromigrating through the capillary tube is indirectly detected and recorded.

REFERENCES:
patent: 4675300 (1987-06-01), Zare et al.
Jorgenson & Lukacs, "Capillary Zone Electrophoresis" Science vol. 222 (Oct. 1983) pp. 266-272.
Roach, Gozel, and Zare "Determinaton of Methotrexate and its Major Metabolite, 7-Hydroxymethotrexate, Using Capillary Zone Electropheresis and Laser-Induced Fluorescence Detection" Journal of Chromatography, 426 (1988) 129-140.
Gozel, Gassmann, and Zare "Electrokinetic Resolution of Amino Acid Enantiomers with Copper(II)-Aspartame Support Electrolyte" Anal. Chem., 1987, 59, 44-49.
Gassmann, Kuo, and Zare, "Electrokinetic Separation of Chiral Compounds" Science (15 Nov. 1985) 813-814.
Burton and Sepaniak "Analysis of B.sub.6 Vitamers by Micellar Electrokinetic Capillary Chromatography with Laser-Excited Fluorescence Detection" Journal of Chromatographic Science vol. 24, (Aug. 1986) 347-351.
Pawliszyn, J. "Nanoliter Volume Sequential Differential Concentration Gradient Detector" Anal. Chem. (1988), 60, 2796-2801.
Bornhop and Dovichi "Simultaneous Laser-Based Refractive Index and Absorance Determinations within Micrometer Diameter Capillary Tubes" Anal. Chem (1987), 59, 1632-1636.

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