MCT1 transporters expressed in blood brain barrier cells

Details MCT1 transporters expressed in blood brain barrier cells MCT1 transporters expressed in blood brain barrier cells

2004-12-30

2008-11-18

Nickol, Gary B. (Department: 1646)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007200, C435S007300, C435S069100, C435S235100, C435S320100, C435S325000, C530S350000, C536S023100, C536S023500

Reexamination Certificate

active

07452680

ABSTRACT:
MCT1 is consistently expressed at high levels in brain microvessel endothelial cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is actively transported by, the MCT1 transporter, and therefore a candidate substrate for crossing the blood brain barrier. The assays are useful in screening for therapeutic, cytotoxic or imaging compounds used in the treatment or diagnosis of neurological diseases.

REFERENCES:
patent: 6489302 (2002-12-01), Wiessler et al.
patent: 2003/0158254 (2003-08-01), Zerangue et al.
patent: 2004/0072746 (2004-04-01), Sullivan et al.
patent: 2005/0032135 (2005-02-01), Zerangue et al.
patent: WO 01/020331 (2001-03-01), None
patent: WO 03/065982 (2003-08-01), None
patent: WO 2004/033655 (2004-04-01), None
Alderman, D.A.; “A Review of Cellulose Ethers in Hydrophilic Matrices for Oral Controlled-Release Dosage Forms”;Int. J. Pharm. Tech. &Prod. Mfr. 5(3):1-9 (1984).
Altschul, S. et al.; “Basic Local Alignment Search Tool”;J. Mol. Biol. 215:403-410 (1990).
Audus, K. et al.; Characterization of an in vitro blood-brain barrier model system for studying drug transport and metabolism,Pharmaceutical Res. 3(2):81-87 (1986)).
Audus, K. et al.; The use of cultured epithelial and endothelial cells for drug transport and metabolism studies,Pharmaceutical Res. 7(5):435-451 (1990)).
Bamba, M. et al.; “Release Mechanisms in Gelforming Sustained Release Preparations”;Int. J. Pharmaceuticals2:307-315 (1979).
Bowman, P. et al.; “Brain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeability”;Ann. Neurol. 14:396-402 (1983).
Brightman and Neuwelt (ed.); Implications of the blood-brain barrier and its manipulation; vol. 1, Plenum Medical, New York, pp. 53-83 (1989).
Cserr, H. et al.,; “Blood-brain interfaces in vertebrates: a comparative approach”;Am. J. Physiol.—Regulatory, Integrative and Comparative Physiology246:277-288 (1984).
During, M. et al.; “Controlled Release of Dopamine from a Polymeric Brain Implant: In Vivo Characterization”;Ann. Neurol. 25:351-356 (1989).
Goldstein, G. et al.; “The Blood-Brain Barrier”;Scientific American255(3):74-83 (1986).
Hanes, J. et al.; “New Advances in Microsphere-Based Single-Dose Vaccines”;Advanced Drug Delivery Reviews28:97-119 (1997).
Henikoff, S. et al.; Amino Acid Substitution Matrices from Protein Blocks;Proc. Natl. Acad. Sci. USA89:10915-19 (1989).
Howard, M. et al.; “Intracerebral Drug Delivery in Rats with Lesion-Induced Memory Deficits”;J. Neurosurg. 71:105-112 (1898—).
Karlin, S. et al.; “Applications and Statistics for Multiple High-Scoring Segments in Molecular Sequences”;Proc. Nat'l. Acad. Sci. USA90:5873-87 (1993).
Langer, R.; “New Methods of Drug Delivery”;Science249:1527-1533 (1990).
Langer, R. et al.; “Chemical and Physical Structure of Polymers as Carriers for Controlled Release of Bioactive Agents: A Review”;J. Macromol. Sci. Rev. Macromol Chem. 23:61-126 (1983).
Levy, R. et al.; “Inhibition of Calcification of Bioprosthetic Heart Valves by Local Controlled-Release Diphosphonate”;Science228:190-192 (1985).
Masereeuw, R. et al.; “In vitro and in vivo transport of zidovudine (AZT) across the blood-brain barrier and the effect of transport inhibitors”;Pharm. Res. 11(2):324-330 (1994).
Meresse, S. et al.; “Bovine brain endothelial cells express tight junctions and monoamine oxidase activity in long-term culture”;J. Neuorchem. 53:1363-1371 (1989).
Needleman, S. et al.; “A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins”;J. Mol. Biol. 48:443-453 (1970).
Pardridge, W. M.; “Receptor-Mediated Peptide Transport Through the Blood-Brain Barrier”; (1986)Endocrine Rev. 7(3):314-330 (1986).
Pardridge, W. M. et al.; “Comparison of in vitro and in vivo models of drug transcytosis through the blood-brain barrier”;J. Pharmacol. Exp. Thera. 253(2):884-891 (1990).
Pearson, W. et al.; “Improved Tools for Biological Sequence Comparison”;Proc. Nat'l. Acad. Sci. USA85:2444-48 (1988).
Smith, T. et al.; “Comparison of Biosequences”;Adv. Appl. Math. 2:482-489 (1981).
Terasaki, T. et al.; “New Approaches to in vitro Models of Blood-Brain Barrier Drug Transport”;Drug Discovery Today8:944-954 (2003).
Froberg et al., “Expression of monocarboxylate transporter MCT1 in normal and neoplastic human CNS tissues,”Neuroreport, 12(4):761-765 (2001).
Gerhart et al., “Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats,”American J. Physiology, 273(1,pt. 1):E207-E213 (1997).
Shen et al., “Evaluation of an immortalized retinal endothelial cell line as an in vitro model for drug transport studies across the blood-retinal barrier,”Pharmaceutical Research, 20(9):1357-1363 (2003).
Smith et al., “Regulation of MCT1 Function by Protein Kinase—A and C Pathways in Cerebral Endothelial Cells,”Society fo Neuroscience Abstract Viewer and Itinerary Planner, abstract No. 581.15 (2002) from 32nd annual meeting of the society for neuroscience, Orlando, Florida Nov. 2-7, 2002.
Tamai et al., “Transporter-mediated permeation of drugs across the blood-brain barrier,”J. Pharmaceutical Sciences, 89(11):1371-1388 (2000).
Terasaki et al., “Conditionally immortalized cell lines as a new in vitro model for the study of barrier functions,”Biological and Pharmaceutical Bulletin, 24(2):111-118 (2001).

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