Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-01-22
1994-08-23
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435193, 4353201, 43525233, 536 232, C12N 922, C12N 910, C12N 1555
Patent
active
053407334
ABSTRACT:
To provide MboI restriction-modification genes and a method for producing MboI restriction enzyme and MboI modification enzyme by using a novel microorganism having, introduced thereinto, plasmids containing said genes. A method for producing MboI restriction enzyme and/or MboI modification enzyme which comprises incubating a microorganism carrying plasmids having, integrated thereinto, MboI restriction-modification genes, and recovering the MboI restriction enzyme and/or MboI modification enzyme thus produced from the culture. It becomes possible to efficiently produce MboI restriction enzyme and/or MboI modification enzyme which are useful in the field of genetic engineering.
REFERENCES:
J. Mol. Biol. 114 pp. 169-179 (1977) "Two Sequence-specific Endonucleases from Moraxella bovis."
Ito Hiroyuki
Kotani Hirokazu
Nakajima Kazuo
Ueno Takashi
Patterson Jr. Charles L.
Takara Shuzo Co. Ltd.
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