MboI restriction-modification genes

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435193, 4353201, 43525233, 536 232, C12N 922, C12N 910, C12N 1555

Patent

active

053407334

ABSTRACT:
To provide MboI restriction-modification genes and a method for producing MboI restriction enzyme and MboI modification enzyme by using a novel microorganism having, introduced thereinto, plasmids containing said genes. A method for producing MboI restriction enzyme and/or MboI modification enzyme which comprises incubating a microorganism carrying plasmids having, integrated thereinto, MboI restriction-modification genes, and recovering the MboI restriction enzyme and/or MboI modification enzyme thus produced from the culture. It becomes possible to efficiently produce MboI restriction enzyme and/or MboI modification enzyme which are useful in the field of genetic engineering.

REFERENCES:
J. Mol. Biol. 114 pp. 169-179 (1977) "Two Sequence-specific Endonucleases from Moraxella bovis."

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