Matrix modification in the electrophoretic separation of nucleic

Details Matrix modification in the electrophoretic separation of nucleic Matrix modification in the electrophoretic separation of nucleic

1992-05-21

1994-10-11

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, G01N 27447

Patent

active

053544420

ABSTRACT:
The present invention is a method for enhancing the separation of polynucleotides or polypeptides of various chain lengths or a mixture of polynucleotides and polypeptides of various chain lengths using electrophoretic processes to sharpen sample component zones and produce maximum resolution of electrophoretically separated macromolecules by modifying the matrix of electrophoretic gel.

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patent: 5059654 (1991-10-01), Hou et al.
patent: 5068176 (1991-11-01), Vijg et al.
patent: 5159049 (1992-10-01), Allen
Bertold v. Radola et al. "Preparation of rehydratable polyacrylamide gels and their application in ultrathin-layer isoelectric focusing" Electrophoresis 1986, 7 (1986) 28-40.
Robert C. Allen and George M. Graves "Rehydratable Gels: A Potential Reference Standard Support for Electrophoresing PCR-Amplified DNA" Biotechnology, vol. 8 (Dec. 1990) 1288-1290.
Allen, R. C. et al. "Tailoring DNA Resolution in Polyacrylamide Gels with Electrochemistry and Pulsed Constant Power Given Jul. 6, 1989", Washington, D.C. (1 page).

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