Matrix controlled method of delayed fluid delivery for assays

Details Matrix controlled method of delayed fluid delivery for assays Matrix controlled method of delayed fluid delivery for assays

1989-04-28

1992-08-04

Housel, James C.

Chemistry: analytical and immunological testing

Including sample preparation

Volumetric liquid transfer

422 58, 422 61, 422100, G01N 3500, G01N 110

Patent

active

051358727

ABSTRACT:
A matrix controlled device having a reservoir having sidewalls and a bottom, and a plurality of conduits positioned in the reservoir and extending through the bottom thereof. Each conduit has an upper opening, a bottom outlet and an optional sidewall opening at a level intermediate the upper level and bottom of the reservoir and the bottom outlet. A flow control matrix plug is positioned in each conduit at a level between the sidewall opening or upper opening, and the bottom outlet. The flow control matrix plug has an internal passageway size which determines the rate at which liquid flows through the plug. By varying the pore density, pore size, dead volume, length, and elevation of the matrix, delay of the flow rate and initial flow of liquid from the respective conduit can be controlled. The size of an upper opening can be reduced to control air flow rates therethrough, and the size and elevation of a sidewall openings can be selected to control liquid flow rates therethrough. The method for delivering a predetermined volume of liquid to a reaction zone is accomplished by introducing liquid into one or more of such conduits, each conduit having a matrix plug therein at a level below the level of liquid introduction. Each matrix individually delays the first release and rate of delivery of the liquid in the conduit.

REFERENCES:
patent: 4549952 (1985-10-01), Columbus
patent: 4855240 (1989-08-01), Rosenstein et al.
patent: 4868129 (1989-09-01), Gibbons et al.
patent: 4923680 (1990-05-01), Nelson
patent: 4960691 (1990-10-01), Gordon et al.

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