Matrix-associating DNA-binding protein, nucleic acids encoding t

Details Matrix-associating DNA-binding protein, nucleic acids encoding t Matrix-associating DNA-binding protein, nucleic acids encoding t

1995-06-06

1997-07-29

Hendricks, Keith D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Nucleoproteins, e.g., chromatin, chromosomal proteins,...

A61K 3817

Patent

active

056523408

ABSTRACT:
The present invention provides a novel human protein, SATB1, that binds matrix/scaffold-associating DNA regions (MARs). The novel human protein, predominantly expressed in the thymus, has an approximate molecular weight of about 85.9 kD. The SATB1 cDNA encodes a 763 amino acid sequence protein that is capable of binding to special AT rich sequences (ATC sequences). The invention further provides antibodies specifically reactive with such protein. Isolated nucleic acids encoding the novel MAR-binding protein are also provided, as well as vectors containing the nucleic acids and recombinant host cells transformed with such vectors. The invention further provides methods of detecting such nucleic acids by contacting a sample with a nucleic acid probe having a nucleotide sequence capable of hybridizing with the isolated nucleic acids of the present invention. Such probes can correspond to the ATC sequences.

REFERENCES:
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Brotherton, Timothy et al., "Avian Nuclear Matrix Proteins Bind Very Tightly to Cellular DNA of the .beta.-Glovin Gene Enhancer in a Tissue-Specific Fashion". Biochem. 30:5845-5850 (1991).
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Dickinson, Liliane A. et al. "A Tissue-Specific MAR/SAR DNA-Binding Protein with Unusual Binding Site Recognition." Cell. 70:631-645 (1992).

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